CSK monoclonal antibody, clone 5F3
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More Files
- More Functions
Western Blot (Cell lysate)
Western blot analysis using CSK monoclonal antibody, clone 5F3 (Cat # MAB10617) against NIH/3T3 (1) , HeLa (2) , COS-7 (3) , Jurkat (4) , Raw246.7 (5) , A-549 (6) , HL-60 (7) and PC-12 (8) cell lysate.
Immunofluorescence
Immunofluorescence analysis of U251 cells using CSK monoclonal antibody, clone 5F3 (Cat # MAB10617) (green) . Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
Flow Cytometry
Flow cytometric analysis of HL-60 cells using CSK monoclonal antibody, clone 5F3 (Cat # MAB10617) (green) and negative control (purple).
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Specification
Product Description
Mouse monoclonal antibody raised against partial recombinant CSK.
Immunogen
Recombinant protein corresponding to human CSK.
Host
Mouse
Theoretical MW (kDa)
50
Reactivity
Human, Monkey, Mouse, Rat
Form
Liquid
Isotype
IgG1
Recommend Usage
ELISA (1:10000)
Western Blot (1:500-1:2000)
Immunofluorescence (1:200-1:1000)
Flow cytometry (1:200-1:400)
The optimal working dilution should be determined by the end user.Storage Buffer
In ascites (0.03% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis using CSK monoclonal antibody, clone 5F3 (Cat # MAB10617) against NIH/3T3 (1) , HeLa (2) , COS-7 (3) , Jurkat (4) , Raw246.7 (5) , A-549 (6) , HL-60 (7) and PC-12 (8) cell lysate.Immunofluorescence
Immunofluorescence analysis of U251 cells using CSK monoclonal antibody, clone 5F3 (Cat # MAB10617) (green) . Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.Enzyme-linked Immunoabsorbent Assay
Flow Cytometry
Flow cytometric analysis of HL-60 cells using CSK monoclonal antibody, clone 5F3 (Cat # MAB10617) (green) and negative control (purple). -
Gene Info — CSK
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Interactome
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Pathway
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Disease
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