CHUK monoclonal antibody, clone 3G12H9
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More Files
- More Functions
Western Blot (Cell lysate)
Western blot analysis using CHUK monoclonal antibody, clone 3G12H9 (Cat # MAB10555) against Raji (1), Jurkat (2), THP-1 (3) and K-562 (4) cell lysate.
Immunofluorescence
Immunofluorescence analysis of NIH/3T3 cells using CHUK monoclonal antibody, clone 3G12H9 (Cat # MAB10555) (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
Flow Cytometry
Flow cytometric analysis of A-549 cells using CHUK monoclonal antibody, clone 3G12H9 (Cat # MAB10555) (blue) and negative control (red).
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Specification
Product Description
Mouse monoclonal antibody raised against partial recombinant CHUK.
Immunogen
Recombinant protein corresponding to human CHUK.
Host
Mouse
Theoretical MW (kDa)
85
Reactivity
Human
Form
Liquid
Isotype
IgG1
Recommend Usage
ELISA (1:10000)
Western Blot (1:500-1:2000)
Immunofluorescence (1:200-1:1000)
Flow cytometry (1:200-1:400)
The optimal working dilution should be determined by the end user.Storage Buffer
In ascites (0.03% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis using CHUK monoclonal antibody, clone 3G12H9 (Cat # MAB10555) against Raji (1), Jurkat (2), THP-1 (3) and K-562 (4) cell lysate.Immunofluorescence
Immunofluorescence analysis of NIH/3T3 cells using CHUK monoclonal antibody, clone 3G12H9 (Cat # MAB10555) (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.Enzyme-linked Immunoabsorbent Assay
Flow Cytometry
Flow cytometric analysis of A-549 cells using CHUK monoclonal antibody, clone 3G12H9 (Cat # MAB10555) (blue) and negative control (red). -
Gene Info — CHUK
Entrez GeneID
1147Gene Name
CHUK
Gene Alias
IKBKA, IKK-alpha, IKK1, IKKA, NFKBIKA, TCF16
Gene Description
conserved helix-loop-helix ubiquitous kinase
Omim ID
600664Gene Ontology
HyperlinkGene Summary
This gene encodes a member of the serine/threonine protein kinase family. The encoded protein, a component of a cytokine-activated protein complex that is an inhibitor of the essential transcription factor NF-kappa-B complex, phosphorylates sites that trigger the degradation of the inhibitor via the ubiquination pathway, thereby activating the transcription factor. [provided by RefSeq
Other Designations
I-kappa-B kinase 1|I-kappa-B kinase-alpha|IKK-a kinase|IkB kinase alpha subunit|Nuclear factor NFkappaB inhibitor kinase alpha|OTTHUMP00000020273|conserved helix-loop ubiquitous kinase
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Interactome
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Pathway
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Disease
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