JUN monoclonal antibody, clone 4H9
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More Files
- More Functions
Western Blot (Cell lysate)
Western blot analysis using JUN monoclonal antibody, clone 4H9 (Cat # MAB10515) against NIH/3T3 cell lysate.
Western Blot (Recombinant protein)
Western blot analysis using JUN monoclonal antibody, clone 4H9 (Cat # MAB10515) against recombinant protein.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical analysis of paraffin-embedded human cervical cancer (A) and human colon cancer (B) tissues using JUN monoclonal antibody, clone 4H9 (Cat # MAB10515) with DAB staining.
Immunofluorescence
Immunofluorescence analysis of HeLa cells using JUN monoclonal antibody, clone 4H9 (Cat # MAB10515) (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
Enzyme-linked Immunoabsorbent Assay
ELISA detection with JUN monoclonal antibody, clone 4H9 (Cat # MAB10515).
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Specification
Product Description
Mouse monoclonal antibody raised against partial recombinant JUN.
Immunogen
Recombinant protein corresponding to human JUN.
Host
Mouse
Theoretical MW (kDa)
43
Reactivity
Human, Mouse
Form
Liquid
Isotype
IgG1
Recommend Usage
ELISA (1:10000)
Western Blot (1:500-1:2000)
Immunohistochemistry (1:200-1:1000)
Immunofluorescence (1:200-1:1000)
The optimal working dilution should be determined by the end user.Storage Buffer
In ascites (0.03% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis using JUN monoclonal antibody, clone 4H9 (Cat # MAB10515) against NIH/3T3 cell lysate.Western Blot (Recombinant protein)
Western blot analysis using JUN monoclonal antibody, clone 4H9 (Cat # MAB10515) against recombinant protein.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical analysis of paraffin-embedded human cervical cancer (A) and human colon cancer (B) tissues using JUN monoclonal antibody, clone 4H9 (Cat # MAB10515) with DAB staining.Immunofluorescence
Immunofluorescence analysis of HeLa cells using JUN monoclonal antibody, clone 4H9 (Cat # MAB10515) (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.Enzyme-linked Immunoabsorbent Assay
ELISA detection with JUN monoclonal antibody, clone 4H9 (Cat # MAB10515). -
Gene Info — JUN
Entrez GeneID
3725Gene Name
JUN
Gene Alias
AP-1, AP1, c-Jun
Gene Description
jun oncogene
Omim ID
165160Gene Ontology
HyperlinkGene Summary
This gene is the putative transforming gene of avian sarcoma virus 17. It encodes a protein which is highly similar to the viral protein, and which interacts directly with specific target DNA sequences to regulate gene expression. This gene is intronless and is mapped to 1p32-p31, a chromosomal region involved in both translocations and deletions in human malignancies. [provided by RefSeq
Other Designations
Jun activation domain binding protein|OTTHUMP00000010036|activator protein 1|enhancer-binding protein AP1|v-jun avian sarcoma virus 17 oncogene homolog|v-jun sarcoma virus 17 oncogene homolog
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Interactome
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Pathway
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Disease
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