AMACR monoclonal antibody, clone 2A10F3
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More Files
- More Functions
Western Blot (Cell lysate)
Western blot analysis of AMACR monoclonal antibody, clone 2A10F3 (Cat # MAB10444) against Jurkat (1) and LNCaP (2) cell lysate.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical analysis of paraffin-embedded human normal prostate tissue (A) and prostate adenocarcinoma tissue (B), showing cytoplasmic localization using AMACR monoclonal antibody, clone 2A10F3 (Cat # MAB10444) with DAB staining.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical analysis of paraffin-embedded human brain cerebellum using AMACR monoclonal antibody, clone 2A10F3 (Cat # MAB10444).
Immunofluorescence
Confocal immunofluorescence analysis of LNCaP cells using AMACR monoclonal antibody, clone 2A10F3 (Cat # MAB10444) (green).
Red: Actin filaments have been labeled with DY-554 phalloidin.
Blue: DRAQ5 fluorescent DNA dye.
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Specification
Product Description
Mouse monoclonal antibody raised against recombinant AMACR.
Immunogen
Recombinant protein corresponding to human AMACR.
Host
Mouse
Theoretical MW (kDa)
42
Reactivity
Human, Mouse
Form
Liquid
Isotype
IgG2b
Recommend Usage
Western Blot (1:500-1:2000)
Immunohistochemistry (1:200-1:1000)
Immunofluorescence (1:200-1:1000)
ELISA (1:10000)
The optimal working dilution should be determined by the end user.Storage Buffer
In ascitic (0.03% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of AMACR monoclonal antibody, clone 2A10F3 (Cat # MAB10444) against Jurkat (1) and LNCaP (2) cell lysate.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical analysis of paraffin-embedded human normal prostate tissue (A) and prostate adenocarcinoma tissue (B), showing cytoplasmic localization using AMACR monoclonal antibody, clone 2A10F3 (Cat # MAB10444) with DAB staining.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical analysis of paraffin-embedded human brain cerebellum using AMACR monoclonal antibody, clone 2A10F3 (Cat # MAB10444).Immunofluorescence
Confocal immunofluorescence analysis of LNCaP cells using AMACR monoclonal antibody, clone 2A10F3 (Cat # MAB10444) (green).
Red: Actin filaments have been labeled with DY-554 phalloidin.
Blue: DRAQ5 fluorescent DNA dye.Enzyme-linked Immunoabsorbent Assay
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Gene Info — AMACR
Entrez GeneID
23600Gene Name
AMACR
Gene Alias
RACE
Gene Description
alpha-methylacyl-CoA racemase
Omim ID
604489Gene Ontology
HyperlinkGene Summary
Alpha-methylacyl-CoA racemase is responsible for the conversion of pristanoyl-CoA and C27-bile acyl-CoAs to their (S)-stereoisomers, which are the only stereoisomers that can be degraded via peroxisomal beta-oxidation.[supplied by OMIM
Other Designations
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Interactome
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Pathway
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Disease
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