PHB monoclonal antibody, clone 5H7
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More Files
- More Functions
Western Blot (Cell lysate)
Western blot analysis using PHB monoclonal antobody, clone 5H7 (Cat # MAB10296) against A-431 (1), MCF-7 (2), Jurkat (3), HeLa (4), HepG2 (5), A-549 (6), NIH/3T3 (7), COS-7 (8) and PC-12 (9) cell lysate.
Western Blot (Recombinant protein)
Western blot analysis using PHB monoclonal antobody, clone 5H7 (Cat # MAB10296) against recombinant human PHB protein.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical analysis of paraffin-embedded human rectum cancer tissue (A), liver cancer tissue (B), lung cancer tissue (C) and stomach cancer tissue (D) using PHB monoclonal antobody, clone 5H7 (Cat # MAB10296) with DAB staining.
Immunofluorescence
Immunofluorescence analysis of NIH/3T3 cells using PHB monoclonal antobody, clone 5H7 (Cat # MAB10296) (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
Flow Cytometry
Flow cytometric analysis of MCF-7 cells using PHB monoclonal antobody, clone 5H7 (Cat # MAB10296) (blue) and negative control (red).
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Specification
Product Description
Mouse monoclonal antibody raised against recombinant PHB.
Immunogen
Recombinant protein corresponding to human PHB.
Host
Mouse
Theoretical MW (kDa)
30
Reactivity
Human, Monkey, Mouse, Rat
Form
Liquid
Isotype
IgG1
Recommend Usage
ELISA (1:10000)
Western Blot (1:500-1:2000)
Immunohistochemistry (1:200-1:1000)
Immunofluorescence (1:200-1:1000)
Flow cytometry (1:200-1:400)
The optimal working dilution should be determined by the end user.Storage Buffer
In ascites (0.03% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis using PHB monoclonal antobody, clone 5H7 (Cat # MAB10296) against A-431 (1), MCF-7 (2), Jurkat (3), HeLa (4), HepG2 (5), A-549 (6), NIH/3T3 (7), COS-7 (8) and PC-12 (9) cell lysate.Western Blot (Recombinant protein)
Western blot analysis using PHB monoclonal antobody, clone 5H7 (Cat # MAB10296) against recombinant human PHB protein.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical analysis of paraffin-embedded human rectum cancer tissue (A), liver cancer tissue (B), lung cancer tissue (C) and stomach cancer tissue (D) using PHB monoclonal antobody, clone 5H7 (Cat # MAB10296) with DAB staining.Immunofluorescence
Immunofluorescence analysis of NIH/3T3 cells using PHB monoclonal antobody, clone 5H7 (Cat # MAB10296) (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.Enzyme-linked Immunoabsorbent Assay
Flow Cytometry
Flow cytometric analysis of MCF-7 cells using PHB monoclonal antobody, clone 5H7 (Cat # MAB10296) (blue) and negative control (red). -
Gene Info — PHB
Entrez GeneID
5245Gene Name
PHB
Gene Alias
PHB1
Gene Description
prohibitin
Omim ID
176705Gene Ontology
HyperlinkGene Summary
Prohibitin is an evolutionarily conserved gene that is ubiquitously expressed. It is thought to be a negative regulator of cell proliferation and may be a tumor suppressor. Mutations in PHB have been linked to sporadic breast cancer. Prohibitin is expressed as two transcripts with varying lengths of 3' untranslated region. The longer transcript is present at higher levels in proliferating tissues and cells, suggesting that this longer 3' untranslated region may function as a trans-acting regulatory RNA. [provided by RefSeq
Other Designations
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Interactome
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Disease
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