PARP1 monoclonal antibody, clone 7A10
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More Files
- More Functions
Western Blot (Cell lysate)
Western blot analysis using PARP1 monoclonal antobody, clone 7A10 (Cat # MAB10291) against Jurkat (1), K-562 (2), HeLa (3), Raji (4),THP-1 (5) and SW620 (6) cell lysate.
Flow Cytometry
Flow cytometric analysis of Jurkat cells using PARP1 monoclonal antobody, clone 7A10 (Cat # MAB10291) (green) and negative control (purple).
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Specification
Product Description
Mouse monoclonal antibody raised against synthetic peptide of PARP1.
Immunogen
A synthetic peptide (conjugated with KLH) corresponding to human PARP1.
Host
Mouse
Theoretical MW (kDa)
117
Reactivity
Human
Form
Liquid
Isotype
IgG1
Recommend Usage
ELISA (1:10000)
Western Blot (1:500-1:2000)
Flow cytometry (1:200-1:400)
The optimal working dilution should be determined by the end user.Storage Buffer
In ascites (0.03% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis using PARP1 monoclonal antobody, clone 7A10 (Cat # MAB10291) against Jurkat (1), K-562 (2), HeLa (3), Raji (4),THP-1 (5) and SW620 (6) cell lysate.Enzyme-linked Immunoabsorbent Assay
Flow Cytometry
Flow cytometric analysis of Jurkat cells using PARP1 monoclonal antobody, clone 7A10 (Cat # MAB10291) (green) and negative control (purple). -
Gene Info — PARP1
Entrez GeneID
142Gene Name
PARP1
Gene Alias
ADPRT, ADPRT1, PARP, PARP-1, PPOL, pADPRT-1
Gene Description
poly (ADP-ribose) polymerase 1
Omim ID
173870Gene Ontology
HyperlinkGene Summary
This gene encodes a chromatin-associated enzyme, poly(ADP-ribosyl)transferase, which modifies various nuclear proteins by poly(ADP-ribosyl)ation. The modification is dependent on DNA and is involved in the regulation of various important cellular processes such as differentiation, proliferation, and tumor transformation and also in the regulation of the molecular events involved in the recovery of cell from DNA damage. In addition, this enzyme may be the site of mutation in Fanconi anemia, and may participate in the pathophysiology of type I diabetes. [provided by RefSeq
Other Designations
ADP-ribosyltransferase (NAD+; poly (ADP-ribose) polymerase)|ADP-ribosyltransferase NAD(+)|OTTHUMP00000035663|poly (ADP-ribose) polymerase family, member 1|poly(ADP-ribose) polymerase|poly(ADP-ribose) synthetase|poly(ADP-ribosyl)transferase
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Interactome
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Pathway
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Disease
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