ITGAM monoclonal antibody, clone MEM-174
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More Files
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Immunoprecipitation
Immunoprecipitation of human CD11b / CD18 heterodimer from the lysate of washed PBMC isolated from healthy donor. Lysate was subjected to affinity column chromatography using ITGAM monoclonal antibody, clone MEM - 174 (Cat # MAB0877) immunosorbent. Eluted immunoprecipitate was resolved on 7.5 % SDS - PAGE and stained with Coomasie Blue.
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Specification
Product Description
Mouse monoclonal antibody raised against native ITGAM.
Immunogen
Native purified ITGAM from human granulocytes.
Host
Mouse
Theoretical MW (kDa)
165
Reactivity
Human
Specificity
This antibody recognizes CD11b antigen (Mac-1), a 165 KDa type Itransmembrane protein mainly expressed on monocytes, granulocytes and NK-cells. The CD11b mediates neutrophil and monocyte interactions with stimulated endothelium.
Form
Liquid
Isotype
IgG2a
Recommend Usage
The optimal working dilution should be determined by the end user.
Storage Buffer
In PBS, pH 7.4 (0.09% sodium azide)
Storage Instruction
Store at 4°C. Do not freeze.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Immunoprecipitation
Immunoprecipitation of human CD11b / CD18 heterodimer from the lysate of washed PBMC isolated from healthy donor. Lysate was subjected to affinity column chromatography using ITGAM monoclonal antibody, clone MEM - 174 (Cat # MAB0877) immunosorbent. Eluted immunoprecipitate was resolved on 7.5 % SDS - PAGE and stained with Coomasie Blue.Flow Cytometry
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Gene Info — ITGAM
Entrez GeneID
3684Gene Name
ITGAM
Gene Alias
CD11B, CR3A, MAC-1, MAC1A, MGC117044, MO1A, SLEB6
Gene Description
integrin, alpha M (complement component 3 receptor 3 subunit)
Omim ID
120980Gene Ontology
HyperlinkGene Summary
This gene encodes the integrin alpha M chain. Integrins are heterodimeric integral membrane proteins composed of an alpha chain and a beta chain. This I-domain containing alpha integrin combines with the beta 2 chain (ITGB2) to form a leukocyte-specific integrin referred to as macrophage receptor 1 ('Mac-1'), or inactivated-C3b (iC3b) receptor 3 ('CR3'). The alpha M beta 2 integrin is important in the adherence of neutrophils and monocytes to stimulated endothelium, and also in the phagocytosis of complement coated particles. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq
Other Designations
antigen CD11b (p170)|complement component receptor 3, alpha|integrin alpha M|macrophage antigen alpha polypeptide|neutrophil adherence receptor alpha-M subunit
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Interactome
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Pathway
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Disease
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