Bad (phospho S155) monoclonal antibody, clone 27AT381
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More Files
- More Functions
Western Blot (Transfected lysate)
Western analysis of cell extracts from 293 cells transfected with Flag-Bad, using Bad (phospho S155) monoclonal antibody, clone 27AT381 (Cat # MAB0113).
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Specification
Product Description
Mouse monoclonal antibody raised against synthetic phosphopeptide of Bad.
Immunogen
Synthetic phosphopeptide (conjugated with KLH) corresponding to residues surrounding S155 of mouse Bad.
Host
Mouse
Reactivity
Human, Mouse
Form
Liquid
Purification
Protein G purification
Isotype
IgG1
Recommend Usage
Western Blot (1:100-500)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.09% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western Blot (Transfected lysate)
Western analysis of cell extracts from 293 cells transfected with Flag-Bad, using Bad (phospho S155) monoclonal antibody, clone 27AT381 (Cat # MAB0113). -
Gene Info — Bad
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Interactome
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Publication Reference
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Suppression of B-cell lymphomagenesis by the BH3-only proteins Bmf and Bad.
Frenzel A, Labi V, Chmelewskij W, Ploner C, Geley S, Fiegl H, Tzankov A, Villunger A.
Blood 2009 Dec; 115(5):995.
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GLP-1 mediates antiapoptotic effect by phosphorylating Bad through a beta-arrestin 1-mediated ERK1/2 activation in pancreatic beta-cells.
Quoyer J, Longuet C, Broca C, Linck N, Costes S, Varin E, Bockaert J, Bertrand G, Dalle S.
The Journal of Biological Chemistry 2009 Nov; 285(3):1989.
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Identification of novel in vivo phosphorylation sites of the human proapoptotic protein BAD: pore-forming activity of BAD is regulated by phosphorylation.
Polzien L, Baljuls A, Rennefahrt UE, Fischer A, Schmitz W, Zahedi RP, Sickmann A, Metz R, Albert S, Benz R, Hekman M, Rapp UR.
The Journal of Biological Chemistry 2009 Aug; 284(41):28004.
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Suppression of B-cell lymphomagenesis by the BH3-only proteins Bmf and Bad.
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