CHUK monoclonal antibody, clone 14A231(MAB0052) | Abnova

CHUK monoclonal antibody, clone 14A231

Catalog # MAB0052

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Size:100 ug

Price: USD $ 638.00

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Western Blot (Cell lysate)

Application

Western Blot (Cell lysate)

Western blot analysis of 30 ug of total cell lysate from Daudi cells with CHUK monoclonal antibody, clone 14A231 (Cat # MAB0052) at 1 ug/mL dilution.

Specification

Product Description

Mouse monoclonal antibody raised against full length recombinant CHUK.

Immunogen

Recombinant His fusion protein corresponding to full length human CHUK.

Host

Mouse

Reactivity

Human, Monkey, Mouse

Form

Liquid

Isotype

IgG1

Recommend Usage

The optimal working dilution should be determined by the end user.

Storage Buffer

In PBS (0.05% BSA, 0.05% sodium azide)

Storage Instruction

Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.

Note

This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Applications

Western Blot (Cell lysate)
Western blot analysis of 30 ug of total cell lysate from Daudi cells with CHUK monoclonal antibody, clone 14A231 (Cat # MAB0052) at 1 ug/mL dilution.

Immunoprecipitation
Gene Info — CHUK

Entrez GeneID
1147

Gene Name

CHUK

Gene Alias

IKBKA, IKK-alpha, IKK1, IKKA, NFKBIKA, TCF16

Gene Description

conserved helix-loop-helix ubiquitous kinase

Omim ID
600664

Gene Ontology
Hyperlink

Gene Summary

This gene encodes a member of the serine/threonine protein kinase family. The encoded protein, a component of a cytokine-activated protein complex that is an inhibitor of the essential transcription factor NF-kappa-B complex, phosphorylates sites that trigger the degradation of the inhibitor via the ubiquination pathway, thereby activating the transcription factor. [provided by RefSeq

Other Designations

I-kappa-B kinase 1|I-kappa-B kinase-alpha|IKK-a kinase|IkB kinase alpha subunit|Nuclear factor NFkappaB inhibitor kinase alpha|OTTHUMP00000020273|conserved helix-loop ubiquitous kinase
Interactome
- CHUK
Pathway
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Publication Reference
- Hepatocyte-specific IKK gamma/NEMO expression determines the degree of liver injury.
  Beraza N, Ludde T, Assmus U, Roskams T, Vander Borght S, Trautwein C.
  
  Gastroenterology 2007 Apr; 132(7):2504.
- An efficient tandem affinity purification procedure for interaction proteomics in mammalian cells.
  Burckstummer T, Bennett KL, Preradovic A, Schutze G, Hantschel O, Superti-Furga G, Bauch A.
  
  Nature Methods 2006 Oct; 3(12):1013.
- Irf6 is a key determinant of the keratinocyte proliferation-differentiation switch.
  Richardson RJ, Dixon J, Malhotra S, Hardman MJ, Knowles L, Boot-Handford RP, Shore P, Whitmarsh A, Dixon MJ.
  
  Nature Genetics 2006 Oct; 38(11):1329.
- Evidence that TNF-TNFR1-TRADD-TRAF2-RIP-TAK1-IKK pathway mediates constitutive NF-kappaB activation and proliferation in human head and neck squamous cell carcinoma.
  Jackson-Bernitsas DG, Ichikawa H, Takada Y, Myers JN, Lin XL, Darnay BG, Chaturvedi MM, Aggarwal BB.
  
  Oncogene 2006 Sep; 26(10):1385.
- Epithelial cell I kappa B-kinase beta has an important protective role in Clostridium difficile toxin A-induced mucosal injury.
  Chae S, Eckmann L, Miyamoto Y, Pothoulakis C, Karin M, Kagnoff MF.
  
  Journal of Immunology 2006 Jul; 177(2):1214.
- Indirubin enhances tumor necrosis factor-induced apoptosis through modulation of nuclear factor-kappa B signaling pathway.
  Gautam Sethi, Kwang Seok Ahn, Santosh K Sandur, Xin Lin, Madan M Chaturvedi, Bharat B Aggarwal.
  
  The Journal of Biological Chemistry 2006 Aug; 281(33):23425.
- Notch3 and pre-TCR interaction unveils distinct NF-kappaB pathways in T-cell development and leukemia.
  Alessandra Vacca, Maria Pia Felli, Rocco Palermo, Giuseppina Di Mario, Angelica Calce, Monica Di Giovine, Luigi Frati, Alberto Gulino, Isabella Screpanti.
  
  The EMBO Journal 2006 Mar; 25(5):1000.
  
  Application：IP, KA, WB-Ce, WB-Tr, Mouse, Mouse thymocytes.
- Skin lesion development in a mouse model of incontinentia pigmenti is triggered by NEMO deficiency in epidermal keratinocytes and requires TNF signaling.
  Arianna Nenci, Marion Huth, Alfred Funteh, Marc Schmidt-Supprian, Wilhelm Bloch, Daniel Metzger, Pierre Chambon, Klaus Rajewsky, Thomas Krieg, Ingo Haase, Manolis Pasparakis.
  
  Human Molecular Genetics 2006 Feb; 15(4):531.
- A point mutation in NEMO associated with anhidrotic ectodermal dysplasia with immunodeficiency pathology results in destabilization of the oligomer and reduces lipopolysaccharide- and tumor necrosis factor-mediated NF-kappa B activation.
  Emilie Vinolo, Helene Sebban, Alain Chaffotte, Alain Israel, Gilles Courtois, Michel Veron, Fabrice Agou.
  
  The Journal of Biological Chemistry 2006 Mar; 281(10):6334.
  
  Application：WB-Tr, Human, 1.3E2, 70Z/3 cells.
- Suberoylanilide hydroxamic acid potentiates apoptosis, inhibits invasion, and abolishes osteoclastogenesis by suppressing nuclear factor-kappaB activation.
  Yasunari Takada, Ann Gillenwater, Haruyo Ichikawa, Bharat B Aggarwal.
  
  The Journal of Biological Chemistry 2006 Mar; 281(9):5612.
- N-(4-hydroxyphenyl)retinamide inhibits invasion, suppresses osteoclastogenesis, and potentiates apoptosis through down-regulation of I(kappa)B(alpha) kinase and nuclear factor-kappaB-regulated gene products.
  Shishir Shishodia, Angelica M Gutierrez, Reuben Lotan, Bharat B Aggarwal.
  
  Cancer Research 2005 Oct; 65(20):9555.
- Curcumin (diferuloylmethane) inhibits constitutive NF-kappaB activation, induces G1/S arrest, suppresses proliferation, and induces apoptosis in mantle cell lymphoma.
  Shishir Shishodia, Hesham M Amin, Raymond Lai, Bharat B Aggarwal.
  
  Biochemical Pharmacology 2005 Sep; 70(5):700.
- Aberrant NF-kappaB2/p52 expression in Hodgkin/Reed-Sternberg cells and CD30-transformed rat fibroblasts.
  Mizuho Nonaka, Ryouichi Horie, Kinji Itoh, Toshiki Watanabe, Naoki Yamamoto, Shoji Yamaoka.
  
  Oncogene 2005 Jun; 24(24):3976.
  
  Application：KA, IP, WB-Ce, Human, H-RS, MT-2 cells.
- Identification of a novel blocker of I kappa B alpha kinase that enhances cellular apoptosis and inhibits cellular invasion through suppression of NF-kappa B-regulated gene products.
  Haruyo Ichikawa, Yasunari Takada, Akira Murakami, Bharat B Aggarwal.
  
  The Journal of Immunology 2005 Jun; 174(11):7383.
- I{kappa}B kinase (IKK){beta}, but not IKK{alpha}, is a critical mediator of osteoclast survival and is required for inflammation-induced bone loss.
  Maria Grazia Ruocco, Shin Maeda, Jin Mo Park, Toby Lawrence, Li-Chung Hsu, Yixue Cao, Georg Schett, Erwin F Wagner, Michael Karin.
  
  The Journal of Experimental Medicine 2005 May; 201(10):1677.
  
  Application：WB-Tr, Mouse, Mouse primary osteoblasts.
- Nurselike cells express BAFF and APRIL, which can promote survival of chronic lymphocytic leukemia cells via a paracrine pathway distinct from that of SDF-1alpha.
  Mitsufumi Nishio, Tomoyuki Endo, Nobuhiro Tsukada, Junko Ohata, Shinichi Kitada, John C Reed, Nathan J Zvaifler, Thomas J Kipps.
  
  Blood 2005 Aug; 106(3):1012.
- Iron-mediated H2O2 production as a mechanism for cell type-specific inhibition of tumor necrosis factor alpha-induced but not interleukin-1beta-induced IkappaB kinase complex/nuclear factor-kappaB activation.
  Andreas Panopoulos, Maged Harraz, John F Engelhardt, Ebrahim Zandi.
  
  The Journal of Biological Chemistry 2005 Jan; 280(4):2912.
  
  Application：KA, WB-Ce, Mouse, MEFs.
- Guggulsterone inhibits NF-kappaB and IkappaBalpha kinase activation, suppresses expression of anti-apoptotic gene products, and enhances apoptosis.
  Shishodia S, Aggarwal BB.
  
  The Journal of Biological Chemistry 2004 Nov; 279(45):47148.
  
  Application：WB, Human, H1299 cells.
- Cyclooxygenase (COX)-2 inhibitor celecoxib abrogates TNF-induced NF-kappa B activation through inhibition of activation of I kappa B alpha kinase and Akt in human non-small cell lung carcinoma: correlation with suppression of COX-2 synthesis.
  Shishodia S, Koul D, Aggarwal BB.
  
  Journal of Immunology 2004 Aug; 173(3):2011.
  
  Application：WB, Human, H1299 cells.
- Genetic deletion of glycogen synthase kinase-3beta abrogates activation of IkappaBalpha kinase, JNK, Akt, and p44/p42 MAPK but potentiates apoptosis induced by tumor necrosis factor.
  Takada Y, Fang X, Jamaluddin MS, Boyd DD, Aggarwal BB.
  
  The Journal of Biological Chemistry 2004 Sep; 279(38):39541.
  
  Application：WB, Mouse, MEFs.
- Protein farnesyltransferase inhibitor (SCH 66336) abolishes NF-kappaB activation induced by various carcinogens and inflammatory stimuli leading to suppression of NF-kappaB-regulated gene expression and up-regulation of apoptosis.
  Yasunari Takada, Fadlo R Khuri, Bharat B Aggarwal.
  
  The Journal of Biological Chemistry 2004 Jun; 279(25):26287.
- Activation of intrinsic and extrinsic proapoptotic signaling pathways in interleukin-18-mediated human cardiac endothelial cell death.
  Bysani Chandrasekar, Kirankumar Vemula, Rama Mohan Surabhi, Min Li-Weber, Laurie B Owen-Schaub, Liselotte E Jensen, Srinivas Mummidi.
  
  The Journal of Biological Chemistry 2004 May; 279(19):20221.
  
  Application：WB-Tr, Human, Human cardiac microvascular endothelial cells.
- Identification of a p65 peptide that selectively inhibits NF-kappa B activation induced by various inflammatory stimuli and its role in down-regulation of NF-kappaB-mediated gene expression and up-regulation of apoptosis.
  Takada Y, Singh S, Aggarwal BB.
  
  The Journal of Biological Chemistry 2004 Apr; 279(15):1096.
  
  Application：IP, WB, Human, HEK 293 cells.
- Flavopiridol inhibits NF-kappaB activation induced by various carcinogens and inflammatory agents through inhibition of IkappaBalpha kinase and p65 phosphorylation: abrogation of cyclin D1, cyclooxygenase-2, and matrix metalloprotease-9.
  Yasunari Takada, Bharat B Aggarwal.
  
  The Journal of Biological Chemistry 2004 Feb; 279(6):4750.
- Two carboxyl-terminal activation regions of Epstein-Barr virus latent membrane protein 1 activate NF-kappaB through distinct signaling pathways in fibroblast cell lines.
  Naohito Saito, Gilles Courtois, Ayako Chiba, Norio Yamamoto, Takeshi Nitta, Noriko Hironaka, Martin Rowe, Naoki Yamamoto, Shoji Yamaoka.
  
  The Journal of Biological Chemistry 2003 Nov; 278(47):46565.
  
  Application：IP, WB-Tr, Rat, 5R, Rat-1 cells.
- Ursolic acid inhibits nuclear factor-kappaB activation induced by carcinogenic agents through suppression of IkappaBalpha kinase and p65 phosphorylation: correlation with down-regulation of cyclooxygenase 2, matrix metalloproteinase 9, and cyclin D1.
  Shishir Shishodia, Sekhar Majumdar, Sanjeev Banerjee, Bharat B Aggarwal.
  
  Cancer Research 2003 Aug; 63(15):4375.
  
  Application：IP, KA, WB-Ce, Human, Jurkat cells.
- The two faces of IKK and NF-kappaB inhibition: prevention of systemic inflammation but increased local injury following intestinal ischemia-reperfusion.
  Lee-Wei Chen, Laurence Egan, Zhi-Wei Li, Florian R Greten, Martin F Kagnoff, Michael Karin.
  
  Nature Medicine 2003 May; 9(5):575.
  
  Application：KA, WB-Ti, WB-Tr, Mouse, Mouse enterocytes, Mouse intestinal epithelial cells, Mouse lungs.
- Adenosine suppresses activation of nuclear factor-kappaB selectively induced by tumor necrosis factor in different cell types.
  Majumdar S, Aggarwal BB.
  
  Oncogene 2003 Feb; 22(8):1206.
  
  Application：WB-Ce, Human, KBM-5 cells.
- Piceatannol inhibits TNF-induced NF-kappaB activation and NF-kappaB-mediated gene expression through suppression of IkappaBalpha kinase and p65 phosphorylation.
  Kazuhiro Ashikawa, Sekhar Majumdar, Sanjeev Banerjee, Alok C. Bharti, Shishir Shishodia, Bharat B. Aggarwal
  
  The Journal of Immunology 2002 Dec; 169(11):6490.
- A recessive mutant cell line with a constitutive IkappaB kinase activity.
  Chinanonwait N, Miura H, Yamamoto N, Yamaoka S.
  
  FEBS Letters 2002 Nov; 531(3):553.
  
  Application：WB-Tr, Rat, Rat-2 fibroblasts.
- The lymphotoxin-beta receptor induces different patterns of gene expression via two NF-kappaB pathways.
  Emmanuel Dejardin, Nathalie M Droin, Mireille Delhase, Elvira Haas, Yixue Cao, Constantin Makris, Zhi-Wei Li, Michael Karin, Carl F Ware, Douglas R Green.
  
  Immunity 2002 Oct; 17(4):525.
  
  Application：IP, KA, WB-Ce, WB-Tr, Human, Mouse, HEK 293T cells, MEFs.
- Cigarette smoke condensate activates nuclear transcription factor-kappaB through phosphorylation and degradation of IkappaB(alpha): correlation with induction of cyclooxygenase-2.
  Anto RJ, Mukhopadhyay A, Shishodia S, Gairola CG, Aggarwal BB.
  
  Carcinogenesis 2002 Sep; 23(9):1511.
  
  Application：KA, WB-Ce, Human, U-937 cells.
- The carboxyl-terminal region of IkappaB kinase gamma (IKKgamma) is required for full IKK activation.
  Constantin Makris, Jaclyn L Roberts, Michael Karin.
  
  Molecular and Cellular Biology 2002 Sep; 22(18):6573.
  
  Application：IP, KA, WB-Ce, WB-Tr, Mouse, Human fibroblasts, MEFs, Mouse fibroblasts.
- Thalidomide suppresses NF-kappa B activation induced by TNF and H2O2, but not that activated by ceramide, lipopolysaccharides, or phorbol ester.
  Sekhar Majumdar, Betty Lamothe, Bharat B Aggarwal.
  
  The Journal of Immunology 2002 Mar; 168(6):2644.
- NEMO trimerizes through its coiled-coil C-terminal domain.
  Fabrice Agou, Fei Ye, Stephane Goffinont, Gilles Courtois, Shoji Yamaoka, Alain Israel, Michel Veron.
  
  The Journal of Biological Chemistry 2002 May; 277(20):17464.
  
  Application：WB-Tr, Mouse, 1.3E2, 70Z/3 cells.
- IKKalpha provides an essential link between RANK signaling and cyclin D1 expression during mammary gland development.
  Cao Y, Bonizzi G, Seagroves TN, Greten FR, Johnson R, Schmidt EV, Karin M.
  
  Cell 2001 Dec; 107(6):763.
  
  Application：WB-Ti, Mouse, Mammary glands.
- Methotrexate suppresses NF-kappaB activation through inhibition of IkappaBalpha phosphorylation and degradation.
  S Majumdar, B B Aggarwal.
  
  The Journal of Immunology 2001 Sep; 167(5):2911.
- Complete reconstitution of human IkappaB kinase (IKK) complex in yeast. Assessment of its stoichiometry and the role of IKKgamma on the complex activity in the absence of stimulation.
  Miller BS, Zandi E.
  
  The Journal of Biological Chemistry 2001 Sep; 276(39):36320.
  
  Application：WB-Ce, WB-Tr, Human, Yeast, HeLa cells, Saccharomyces cerevisiae.
- Tumor suppressor MMAC/PTEN inhibits cytokine-induced NFkappaB activation without interfering with the IkappaB degradation pathway.
  Koul D, Yao Y, Abbruzzese JL, Yung WK, Reddy SA.
  
  The Journal of Biological Chemistry 2001 Apr; 276(14):11402.
  
  Application：WB-Ce, Human, U251, U251(MMAC) cells.
- Leflunomide suppresses TNF-induced cellular responses: effects on NF-kappa B, activator protein-1, c-Jun N-terminal protein kinase, and apoptosis.
  S K Manna, A Mukhopadhyay, B B Aggarwal.
  
  The Journal of Immunology 2000 Nov; 165(10):5962.
- IFN-alpha suppresses activation of nuclear transcription factors NF-kappa B and activator protein 1 and potentiates TNF-induced apoptosis.
  S K Manna, A Mukhopadhyay, B B Aggarwal.
  
  Journal of Immunology 2000 Nov; 165(9):4927.
  
  Application：IP, KA, WB-Ce, Human, Jurkat cells.

Contact Info

+1-909-264-1399
+1-909-992-0619
Toll Free : +1-877-853-6098
+1-909-992-3401
sales@abnova.com

Product Inquiry

Service Inquiry