AGR2 HEK293 Cell Transient Overexpression Lysate(Non-Denatured)
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More Files
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Specification
Transfected Cell Line
HEK293
Plasmid
pCMV-AGR2 full length
Host
Human
Theoretical MW (kDa)
20
Lysis Buffer
Modified RIPA Lysis Buffer:50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF.
Concentration
1.9 mg/ml
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-AGR2 antibody (H00010551-M03) by Western Blots.
SDS-PAGE Gel
AGR2 transfected lysate
Western Blot
Lane 1: AGR2 transfected lysate ( 20 KDa).
Lane 2: Non-transfected lysate.Recommend Usage
Use it directly for immuno-precipitation, or heat lysate with SDS gel loading buffer to 95°C for 5 minutes followed by rapid cooling for western blot application. If dissociating conditions are required, add reducing agent prior to heating.
Storage Buffer
In modified RIPA Lysis Buffer.
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
Immunoprecipitation
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Gene Info — AGR2
Entrez GeneID
10551GeneBank Accession#
BC015503Protein Accession#
AAH15503Gene Name
AGR2
Gene Alias
AG2, GOB-4, HAG-2, XAG-2
Gene Description
anterior gradient homolog 2 (Xenopus laevis)
Omim ID
606358Gene Ontology
HyperlinkOther Designations
anterior gradient 2 homolog|secreted cement gland homolog
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Interactome
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Disease
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