mutaFISH™ RNA Accessory Kit
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More Files
- More Functions
mutation specific, Fluorescence In Situ Hybridization (Cells)
The figure represents an example workflow of detecting a point mutation on single strand RNA using mutaFISH™ probes with mutaFISH™ RNA Accessory Kit.
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Specification
Product Description
mutaFISH™ RNA Accessory Kit provides reagents and enzymes for in situ reverse transcription, RNA digestion, mutaFISH™ hybridization, ligation and amplication prior to mutaFISH™.
Supplied Product
Box 1 content:
1. DEPC-H2O
2. DEPC-PBS
3. PBS-T
4. Permeabilization Buffer
5. Formamide
6. Detection Buffer
Box 2 content:
1. 3% Paraformaldehyde
2. dNTP Mix
3. BSA
4. RNase Inhibitor
5. RT Enzyme
6. 5X RT Buffer
7. Hybrid Enzyme
8. 10X Hybrid Enzyme Buffer
9. RNase H
10. 1M KCL
11. DNA Polymerase
12. 10X DNA Polymerase Buffer
13. 50% Glycerol
14. DAPI (100uM)Comparison
Regulatory Status
For research use only (RUO)
Storage Instruction
Store Box 1 at 4°C, store Box 2 at -20°C.
Video
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Applications
mutation specific, Fluorescence In Situ Hybridization (Cells)
The figure represents an example workflow of detecting a point mutation on single strand RNA using mutaFISH™ probes with mutaFISH™ RNA Accessory Kit.
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