CytoQuest™ Universal Cancer Stem Cell CSV CSV CD45 Antibody Kit
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More Files
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Immunofluorescence (Circulating Cancer Stem Cell)
Representative images of EMT transformed CSC (red arrow) and WBC from pancreatic cancer patient. CSV EMT CSCs were detected by using immunofluorescence staining for CSV (FITC, green), CD133 (Alexa647, red), CD45 (PE, orange) and Nucleus (DAPI, blue).
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Specification
Product Description
CytoQuest™ Universal Cancer Stem Cell CSV CSV CD45 Antibody Kit contains antibodies for immobilization and immunostaining of cancer stem cells.
Instrument Requirement
Chip Requirement
Supplied Product
it content:
1. Anti-CSV capturing antibody (Biotin):
Biotin conjugated Anti-CSV antibody for cancer stem cell capturing.
2. Anti-CSV detecting antibody (FITC):
FITC conjugated Anti-CSV antibody for cancer stem cell detection.
3. Anti-CD45 detecting antibody (PE):
PE conjugated Anti-CD45 antibody for cancer stem cell detection.
4. 50X Antibody Dilution Buffer (50X ADB).
*Reagents are sufficient for 20 assays using recommended protocol.Regulatory Status
For research use only (RUO)
Storage Instruction
Store Anti-CSV capturing antibody (Biotin), Anti-CSV detecting antibody (FITC) and Anti-CD45 detecting antibody (PE) at 4°C.
Store 50X Antibody Dilution Buffer (50X ADB) at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
Cell-Surface Vimentin (CSV) detecting antibody is best used before cell fixation and permeabilization. If fixation is required, please use Abnova’s Special Fixative.
Cell-Surface Vimentin (CSV) antibody is a pending MD Anderson patent which has been exclusively licensed to Abnova Corporation. -
Applications
Immunofluorescence (Circulating Cancer Stem Cell)
Representative images of EMT transformed CSC (red arrow) and WBC from pancreatic cancer patient. CSV EMT CSCs were detected by using immunofluorescence staining for CSV (FITC, green), CD133 (Alexa647, red), CD45 (PE, orange) and Nucleus (DAPI, blue).
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