CytoQuest™ EpCAM PanCK CD45 Mouse Antibody Kit
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Specification
Product Description
CytoQuest™ EpCAM PanCK CD45 Mouse Antibody Kit contains antibodies for immobilization and immunostaining of circulating epithelial cells.
Instrument Requirement
Chip Requirement
Supplied Product
Kit content:
1. Anti-EpCAM capturing antibody (Biotin):
Biotin conjugated Anti-EpCAM antibody for CTC capturing.
2. Anti-PanCK detecting antibody (FITC):
FITC conjugated Anti-PanCK antibody for CTC detection.
3. Anti-Mouse CD45 detecting antibody (PE):
PE conjugated Anti-CD45 antibody for CTC detection.
4. 50X Antibody Dilution Buffer (50X ADB).
*Reagents are sufficient for 20 assays using recommended protocol.Regulatory Status
For research use only (RUO)
Storage Instruction
Store Anti-EpCAM capturing antibody (Biotin), Anti-PanCK detecting antibody (FITC) and Anti-Mouse CD45 detecting antibody (PE) at 4°C.
Store 50X Antibody Dilution Buffer (50X ADB) at -20°C.
Aliquot to avoid repeated freezing and thawing. -
Applications
Immunofluorescence (Circulating Tumor Cell)
Representative images of lung cancer cell (white arrow) and mouse WBC (yellow arrow) from mouse blood spiked with human lung adenocarcinoma cells (PC-9). Lung cancer cells were detected by using immunofluorescence staining for human PanCK (FITC, green), mouse CD45 (PE, orange) and Nucleus (DAPI, blue).
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