AKT/PKB Kinase (Human) Assay/Inhibitor Screening Kit
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More Files
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Specification
Product Description
AKT/PKB Kinase (Human) Assay/Inhibitor Screening Kit is a single-site, non-quantitative immunoassay for AKT activity. Plates are pre-coated with "AKTide-2T" (10), which can be efficiently phosphorylated by AKT1, 2 and 3 on a microtiter plate. The detector antibody is AT-3E2, an antibody that specifically detects only the phosphorylated "AKTide-2T".
Assay Type
Quantitative
Reactivity
Human
Regulation Status
For research use only (RUO)
Quality Control Testing
Dose dependent curve
Dose dependent curve of recombinant AKT enzyme reaction.
Storage Instruction
Store the kit at 4°C.
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Applications
Functional Study
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Gene Info — AKT1
Entrez GeneID
207Gene Name
AKT1
Gene Alias
AKT, MGC99656, PKB, PKB-ALPHA, PRKBA, RAC, RAC-ALPHA
Gene Description
v-akt murine thymoma viral oncogene homolog 1
Gene Ontology
HyperlinkGene Summary
The serine-threonine protein kinase encoded by the AKT1 gene is catalytically inactive in serum-starved primary and immortalized fibroblasts. AKT1 and the related AKT2 are activated by platelet-derived growth factor. The activation is rapid and specific, and it is abrogated by mutations in the pleckstrin homology domain of AKT1. It was shown that the activation occurs through phosphatidylinositol 3-kinase. In the developing nervous system AKT is a critical mediator of growth factor-induced neuronal survival. Survival factors can suppress apoptosis in a transcription-independent manner by activating the serine/threonine kinase AKT1, which then phosphorylates and inactivates components of the apoptotic machinery. Multiple alternatively spliced transcript variants have been found for this gene. [provided by RefSeq
Other Designations
RAC-alpha serine/threonine-protein kinase|murine thymoma viral (v-akt) oncogene homolog-1|protein kinase B|rac protein kinase alpha
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Interactome
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Pathway
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Disease
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Publication Reference
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Reversal of global apoptosis and regional stress kinase activation by cardiac resynchronization.
Chakir K, Daya SK, Tunin RS, Helm RH, Byrne MJ, Dimaano VL, Lardo AC, Abraham TP, Tomaselli GF, Kass DA.
Circulation 2008 Mar; 117(11):1369.
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Asbestos induces nitric oxide synthesis in mesothelioma cells via Rho signaling inhibition.
Riganti C, Orecchia S, Silvagno F, Pescarmona G, Betta PG, Gazzano E, Aldieri E, Ghigo D, Bosia A.
American Journal of Respiratory Cell and Molecular Biology 2007 Jun; 36(6):746.
Application:Func, Human, HMM (MM98) cells.
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Reversal of global apoptosis and regional stress kinase activation by cardiac resynchronization.
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