CENTD2 293T Cell Transient Overexpression Lysate(Denatured)
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More Files
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SDS-PAGE Gel
CENTD2 transfected lysate.
Western Blot
Lane 1: CENTD2 transfected lysate ( 135.8 KDa)
Lane 2: Non-transfected lysate.
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Specification
Transfected Cell Line
293T
Plasmid
pCMV-CENTD2 full-length
Host
Human
Theoretical MW (kDa)
135.8
Interspecies Antigen Sequence
Mouse (91); Rat (91)
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-CENTD2 antibody (H00116985-B01) by Western Blots.
SDS-PAGE Gel
CENTD2 transfected lysate.
Western Blot
Lane 1: CENTD2 transfected lysate ( 135.8 KDa)
Lane 2: Non-transfected lysate.Storage Buffer
1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
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Gene Info — ARAP1
Entrez GeneID
116985GeneBank Accession#
NM_139181Protein Accession#
NP_631920.1Gene Name
ARAP1
Gene Alias
CENTD2, KIAA0782
Gene Description
ArfGAP with RhoGAP domain, ankyrin repeat and PH domain 1
Omim ID
606646Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene contains SAM, ARF-GAP, RHO-GAP, ankyrin repeat, RAS-associating, and pleckstrin homology (PH) domains. In vitro, this protein displays RHO-GAP and phosphatidylinositol (3,4,5) trisphosphate (PIP3)-dependent ARF-GAP activity. The encoded protein associates with the Golgi, and the ARF-GAP activity mediates changes in the Golgi and the formation of filopodia. It is thought to regulate the cell-specific trafficking of a receptor protein involved in apoptosis. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq
Other Designations
ARF-GAP, RHO-GAP, ankyrin repeat, and pleckstrin homology domains-containing protein 1|centaurin, delta 2
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Interactome
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