Products

Product Description

Purified CD274 (AAH74984.1, 18 a.a. - 238 a.a.) human recombinant protein with His-Flag-StrepII tag at N-terminus expressed in human cells.Recombinant Human Protein,Recombinant Human Proteins,Human Recombinant Protein,Human Recombinant Proteins,HuPro

Transfected Cell Line

Human HEK293H cells

Sequence

AFTVTVPKDLYVVEYGSNMTIECKFPVEKQLDLAALIVYWEMEDKNIIQFVHGEEDLKVQHSSYRQRARLLKDQLSLGNAALQITDVKLQDAGVYRCMISYGGADYKRITVKVNAPYNKINQRILVVDPVTSEHELTCQAEGYPKAEVIWTSSDHQVLSGKTTTTNSKREEKLFNVTSTLRINTTTNEIFYCTFRRLDPEENHTAELVIPELPLAHPPNER

Host

Human

Theoretical MW (kDa)

29.59

Interspecies Antigen Sequence

Mouse (69); Rat (69)

Form

Liquid

Preparation Method

Transfection of pSuper-CD274 plasmid into HEK293H cell, and the expressed protein was purified by Strep-Tactin affinity column.

Purification

Strep-Tactin affinity columns

Concentration

≥ 10 ug/ml

Quality Control Testing

SDS-PAGE and Western Blot

SDS-PAGE Gel

Western Blot

Storage Buffer

100 mM Tris-HCl pH 8.0, 150 mM NaCl, 1 mM EDTA, and 5 mM desthiobiotin.

Storage Instruction

Store at -80°C. Aliquot to avoid repeated freezing and thawing.