BSCL2 293T Cell Transient Overexpression Lysate(Denatured)
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More Files
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SDS-PAGE Gel
BSCL2 transfected lysate.
Western Blot
Lane 1: BSCL2 transfected lysate ( 44.5 KDa)
Lane 2: Non-transfected lysate.
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Specification
Transfected Cell Line
293T
Plasmid
pCMV-BSCL2 full-length
Host
Human
Theoretical MW (kDa)
44.5
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-BSCL2 antibody (H00026580-B01) by Western Blots.
SDS-PAGE Gel
BSCL2 transfected lysate.
Western Blot
Lane 1: BSCL2 transfected lysate ( 44.5 KDa)
Lane 2: Non-transfected lysate.Storage Buffer
1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
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Gene Info — BSCL2
Entrez GeneID
26580GeneBank Accession#
BC009866.2Protein Accession#
AAH09866.2Gene Name
BSCL2
Gene Alias
GNG3LG, HMN5, MGC4694, SPG17
Gene Description
Bernardinelli-Seip congenital lipodystrophy 2 (seipin)
Gene Ontology
HyperlinkGene Summary
This gene encodes protein seipin, which is located in the endoplasmic reticulum and may be important for lipid droplet morphology. Mutations in this gene have been associated with congenital generalized lipodystrophy type 2 or Berardinelli-Seip syndrome, a rare autosomal recessive disease characterized by a near absence of adipose tissue and severe insulin resistance. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq
Other Designations
seipin
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Interactome
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Disease
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