KDM5B monoclonal antibody (M02), clone 1G10
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More Files
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunoperoxidase of monoclonal antibody to KDM5B on formalin-fixed paraffin-embedded human colon. [antibody concentration 1 ug/ml]
Sandwich ELISA (Recombinant protein)
Detection limit for recombinant GST tagged KDM5B is approximately 0.03ng/ml as a capture antibody.
Immunofluorescence
Immunofluorescence of monoclonal antibody to KDM5B on HeLa cell . [antibody concentration 10 ug/ml]
Western Blot detection against Immunogen (35.53 KDa) .
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Specification
Product Description
Mouse monoclonal antibody raised against a partial recombinant KDM5B.
Immunogen
KDM5B (NP_006609, 231 a.a. ~ 319 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Sequence
AKRMRAEAMNIKIEPEETTEARTHNLRRRMGCPTPKCENEKEMKSSIKQEPIERKDYIVENEKEKPKSRSKKATNAVDLYVCLLCGSGN
Host
Mouse
Reactivity
Human
Interspecies Antigen Sequence
Mouse (94); Rat (93)
Isotype
IgG2a Kappa
Quality Control Testing
Antibody Reactive Against Recombinant Protein.
Western Blot detection against Immunogen (35.53 KDa) .
Storage Buffer
In 1x PBS, pH 7.4
Storage Instruction
Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot (Recombinant protein)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunoperoxidase of monoclonal antibody to KDM5B on formalin-fixed paraffin-embedded human colon. [antibody concentration 1 ug/ml]Sandwich ELISA (Recombinant protein)
Detection limit for recombinant GST tagged KDM5B is approximately 0.03ng/ml as a capture antibody.ELISA
Immunofluorescence
Immunofluorescence of monoclonal antibody to KDM5B on HeLa cell . [antibody concentration 10 ug/ml] -
Gene Info — KDM5B
Entrez GeneID
10765GeneBank Accession#
NM_006618Protein Accession#
NP_006609Gene Name
KDM5B
Gene Alias
FLJ10538, FLJ12459, FLJ12491, FLJ16281, FLJ23670, KDM5B, PLU-1, PUT1, RBBP2H1A
Gene Description
jumonji, AT rich interactive domain 1B
Omim ID
605393Gene Ontology
HyperlinkGene Summary
O
Other Designations
OTTHUMP00000038815|RBP2-like|putative DNA/chromatin binding motif|retinoblastoma-binding protein 2, homolog 1A
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Interactome
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Disease
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Publication Reference
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The KDM5B and KDM1A lysine demethylases cooperate in regulating androgen receptor expression and signalling in prostate cancer.
Veronika M Metzler, Simone de Brot, Daisy B Haigh, Corinne L Woodcock, Jennifer Lothion-Roy, Anna E Harris, Emeli M Nilsson, Atara Ntekim, Jenny L Persson, Brian D Robinson, Francesca Khani, Kristian B Laursen, Lorraine J Gudas, Michael S Toss, Srinivasan Madhusudan, Emad Rakha, David M Heery, Catrin S Rutland, Nigel P Mongan, Jennie N Jeyapalan.
Frontiers in Cell and Developmental Biology 2023 Apr; 11:1116424.
Application：IHC, Human, prostate.
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Overexpression of KDM5B/JARID1B is associated with poor prognosis in hepatocellular carcinoma.
Shigekawa Y, Hayami S, Ueno M, Miyamoto A, Suzaki N, Kawai M, Hirono S, Okada KI, Hamamoto R, Yamaue H.
Oncotarget 2018 Sep; 9(76):34320.
Application：IHC, Human, Human hepatocellular carcinoma, HLF, HepG2, Huh7 cells.
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Jumonji/ARID1 B (JARID1B) Protein Promotes Breast Tumor Cell Cycle Progression through Epigenetic Repression of MicroRNA let-7e.
Mitra D, Das PM, Huynh FC, Jones FE.
The Journal of Biological Chemistry 2011 Nov; 286(4):40531.
Application：IP-WB, Human, MCF-7, T47D cells.
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Overexpression of the JmjC histone demethylase KDM5B in human carcinogenesis: involvement in the proliferation of cancer cells through the E2F/RB pathway.
Hayami S, Yoshimatsu M, Veerakumarasivam A, Unoki M, Iwai Y, Tsunoda T, Field HI, Kelly JD, Neal DE, Yamaue H, Ponder BA, Nakamura Y, Hamamoto R.
Molecular Cancer 2010 Mar; 9:59.
Application：IHC, WB, IF, Human, Bladder, lung tumor, A549 and SBC5 cells.
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JARID1B is a histone H3 lysine 4 demethylase up-regulated in prostate cancer.
Xiang Y, Zhu Z, Han G, Ye X, Xu B, Peng Z, Ma Y, Yu Y, Lin H, Chen AP, Chen CD.
PNAS 2007 Nov; 104(49):19226.
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The KDM5B and KDM1A lysine demethylases cooperate in regulating androgen receptor expression and signalling in prostate cancer.
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