STAM monoclonal antibody (M01), clone 2B11-1G1
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More Files
- More Functions
Western Blot (Transfected lysate)
Western Blot analysis of STAM expression in transfected 293T cell line by STAM monoclonal antibody (M01), clone 2B11-1G1.
Lane 1: STAM transfected lysate(45 KDa).
Lane 2: Non-transfected lysate.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunoperoxidase of monoclonal antibody to STAM on formalin-fixed paraffin-embedded human small Intestine. [antibody concentration 3 ug/ml]
Immunoprecipitation
Immunoprecipitation of STAM transfected lysate using anti-STAM monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with STAM monoclonal antibody.
Sandwich ELISA (Recombinant protein)
Detection limit for recombinant GST tagged STAM is 0.1 ng/ml as a capture antibody.
Western Blot detection against Immunogen (70.07 KDa) .
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Specification
Product Description
Mouse monoclonal antibody raised against a full length recombinant STAM.
Immunogen
STAM (AAH30586, 1 a.a. ~ 403 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Sequence
MPLFATNPFDQDVEKATSEMNTAEDWGLILDICDKVGQSRTGPKDCLRSIMRRVNHKDPHVAMQALTLLGACVSNCGKIFHLEVCSRDFASEVSNVLNKGHPKVCEKLKALMVEWTDEFKNDPQLSLISAMIKNLKEQGVTFPAIGSQAAEQAKASPALVAKDPGTVANKKEEEDLAKAIELSLKEQRQQSTTLSTLYPSTSSLLTNHQHEGRKVRAIYDFEAAEDNELTFKAGEIITVLDDSDPNWWKGETHQGIGLFPSNFVTAYLTAEPEMIKTEKKTVQFSDDVQVETIEPEPEPAFIDEDKMDQLLQMLQSTDPSDDQPDLPELLHLEAMCHQMGPLIDEKLEDIDRKHSELSELNVKVMEALSLYTKLMNEDPMYSMYAKLQNQPGSGPTIRKPSPS
Host
Mouse
Reactivity
Human
Isotype
IgG1 Kappa
Quality Control Testing
Antibody Reactive Against Recombinant Protein.
Western Blot detection against Immunogen (70.07 KDa) .
Storage Buffer
In 1x PBS, pH 7.4
Storage Instruction
Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot (Transfected lysate)
Western Blot analysis of STAM expression in transfected 293T cell line by STAM monoclonal antibody (M01), clone 2B11-1G1.
Lane 1: STAM transfected lysate(45 KDa).
Lane 2: Non-transfected lysate.
Western Blot (Recombinant protein)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunoperoxidase of monoclonal antibody to STAM on formalin-fixed paraffin-embedded human small Intestine. [antibody concentration 3 ug/ml]Immunoprecipitation
Immunoprecipitation of STAM transfected lysate using anti-STAM monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with STAM monoclonal antibody.Sandwich ELISA (Recombinant protein)
Detection limit for recombinant GST tagged STAM is 0.1 ng/ml as a capture antibody.ELISA
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Gene Info — STAM
Entrez GeneID
8027GeneBank Accession#
BC030586Protein Accession#
AAH30586Gene Name
STAM
Gene Alias
DKFZp686J2352, STAM1
Gene Description
signal transducing adaptor molecule (SH3 domain and ITAM motif) 1
Omim ID
601899Gene Ontology
HyperlinkGene Summary
This gene was identified by the rapid tyrosine-phosphorylation of its product in response to cytokine stimulation. The encoded protein contains a SH3 domain and the immunoreceptor tyrosine-based activation motif (ITAM). This protein associates with JAK3 and JAK2 kinases via its ITAM region, and is phosphorylated by the JAK kinases upon cytokine stimulation, which suggests the function of this protein is as an adaptor molecule involved in the downstream signaling of cytokine receptors. HGS/HRS (hepatocyte grwoth factor-regulated tyrosine kinase substrate) has been found to bind and counteract the function of this protein. [provided by RefSeq
Other Designations
OTTHUMP00000019237|signal transducing adaptor molecule 1
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Interactome
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Pathway
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Disease
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