ZNF143 monoclonal antibody (M01), clone 2B4
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More Files
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Specification
Product Description
Mouse monoclonal antibody raised against a full length recombinant ZNF143.
Immunogen
ZNF143 (AAH20219, 1 a.a. ~ 626 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Sequence
MTEFPGGGMEAQHVTLCLTEAVTVADGDNLENMEGVSLQAVTLADGSTAYIQHNSKDAKLIDGQVIQLEDGSAAYVQHVPIPKSTGDSLRLEDGQAVQLEDGTTAFIHHTSKDSYDQSALQAVQLEDGTTAYIHHAVQVPQSDTILAIQADGTVAGLHTGDATIDPDTISALEQYAAKVSIDGSESVAGTGMIGENEQEKKMQIVLQGHATRVTAKSQQSGEKAFRCEYDGCGKLYTTAHHLKVHERSHTGDRPYQCEHAGCGKAFATGYGLKSHVRTHTGEKPYRCSEDNCTKSFKTSGDLQKHIRTHTGERPFKCPFEGCGRSFTTSNIRKVHVRTHTGERPYYCTEPGCGRAFASATNYKNHVRIHTGEKPYVCTVPGCDKRFTEYSSLYKHHVVHTHSKPYNCNHCGKTYKQISTLVMHKRTAHNDTEPIEEEQEAFFEPPPGQGEDVLKGSQITYVTGVEGDDVVSTQVATVTQSGLSQQVTLISQDGTQHVNISQADMQAIGNTITMVTQDGTPITVPAHDAVISSAGTHSVAMVTAEGTEGQQVAIVAQDLAAFHTASSEMGHQQHSHHLVTTETRPLTLVATSNGTQIAVQLGEQPSLEEAIRIASRIQQGETPGLDD
Host
Mouse
Reactivity
Human
Interspecies Antigen Sequence
Mouse (97); Rat (97)
Isotype
IgG1 Kappa
Quality Control Testing
Antibody Reactive Against Recombinant Protein.
Western Blot detection against Immunogen (94.6 KDa) .
Storage Buffer
In 1x PBS, pH 7.4
Storage Instruction
Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot (Cell lysate)
ZNF143 monoclonal antibody (M01), clone 2B4 Western Blot analysis of ZNF143 expression in Hela S3 NE ( Cat # L013V3 ).Western Blot (Recombinant protein)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunoperoxidase of monoclonal antibody to ZNF143 on formalin-fixed paraffin-embedded human ovary, clear cell carcinoma tissue. [antibody concentration 3 ug/ml]Sandwich ELISA (Recombinant protein)
Detection limit for recombinant GST tagged ZNF143 is approximately 0.1ng/ml as a capture antibody.ELISA
Immunofluorescence
Immunofluorescence of monoclonal antibody to ZNF143 on HeLa cell. [antibody concentration 10 ug/ml] -
Gene Info — ZNF143
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Interactome
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Publication Reference
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Hi-TrAC reveals division of labor of transcription factors in organizing chromatin loops.
Shuai Liu, Yaqiang Cao, Kairong Cui, Qingsong Tang, Keji Zhao.
Nature Communications 2022 Nov; 13(1):6679.
Application:ChIP-Seq, WB-Tr, Human, K562 cells.
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Distinct and overlapping DNMT1 interactions with multiple transcription factors in erythroid cells: Evidence for co-repressor functions.
Papageorgiou DN, Karkoulia E, Amaral-Psarris A, Burda P, Kolodziej K, Demmers J, Bungert J, Stopka T, Strouboulis J.
Biochimica et Biophysica Acta 2016 Sep; 1859(12):1515.
Application:IP-WB, Human, MEL, HEK 293, K562 cells.
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Genomic Determinants of THAP11/ZNF143/HCFC1 Complex Recruitment to Chromatin.
Vinckevicius A, Parker JB, Chakravarti D.
Molecular and Cellular Biology 2015 Dec; 35(24):4135.
Application:ChIP, Human, SW620 cells.
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Zfp143 regulates Nanog through modulation of Oct4 binding.
Chen X, Fang F, Liou YC, Ng HH.
Stem Cells 2008 Aug; 26(11):2759.
Application:ChIP, WB, Mouse, ES cells (mouse).
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Hi-TrAC reveals division of labor of transcription factors in organizing chromatin loops.
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