VRK1 (Human) IP-WB Antibody Pair
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More Files
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Immunoprecipitation of VRK1 transfected lysate using rabbit polyclonal anti-VRK1 and Protein A Magnetic Bead (U0007), and immunoblotted with mouse purified polyclonal anti-VRK1.
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Specification
Product Description
This IP-WB antibody pair set comes with one antibody for immunoprecipitation and another to detect the precipitated protein in western blot.
Reactivity
Human
Quality Control Testing
Immunoprecipitation-Western Blot (IP-WB)
Immunoprecipitation of VRK1 transfected lysate using rabbit polyclonal anti-VRK1 and Protein A Magnetic Bead (U0007), and immunoblotted with mouse purified polyclonal anti-VRK1.
Supplied Product
Antibody pair set content:
1. Antibody pair for IP: rabbit polyclonal anti-VRK1 (300 ul)
2. Antibody pair for WB: mouse purified polyclonal anti-VRK1 (50 ug)
Storage Instruction
Store reagents of the antibody pair set at -20°C or lower. Please aliquot to avoid repeated freeze thaw cycle. Reagents should be returned to -20°C storage immediately after use.
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Applications
Immunoprecipitation-Western Blot
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Gene Info — VRK1
Entrez GeneID
7443Gene Name
VRK1
Gene Alias
MGC117401, MGC138280, MGC142070
Gene Description
vaccinia related kinase 1
Omim ID
602168Gene Ontology
HyperlinkGene Summary
This gene encodes a member of the vaccinia-related kinase (VRK) family of serine/threonine protein kinases. This gene is widely expressed in human tissues and has increased expression in actively dividing cells, such as those in testis, thymus, fetal liver, and carcinomas. Its protein localizes to the nucleus and has been shown to promote the stability and nuclear accumulation of a transcriptionally active p53 molecule and, in vitro, to phosphorylate Thr18 of p53 and reduce p53 ubiquitination. This gene, therefore, may regulate cell proliferation. This protein also phosphorylates histone, casein, and the transcription factors ATF2 (activating transcription factor 2) and c-JUN. [provided by RefSeq
Other Designations
vaccinia virus B1R-related kinase 1|vaccinia-related kinase-1
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