TNF monoclonal antibody (M02), clone 1C3-A1-F4
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More Files
- More Functions
Western Blot (Tissue lysate)
TNF monoclonal antibody (M02), clone S9. Western Blot analysis of TNF expression in rat testis.
Sandwich ELISA (Recombinant protein)
Detection limit for recombinant GST tagged TNF is approximately 0.03ng/ml as a capture antibody.
Western Blot detection against Immunogen (51.37 KDa) .
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Specification
Product Description
Mouse monoclonal antibody raised against a full length recombinant TNF.
Immunogen
TNF (AAH28148, 1 a.a. ~ 233 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Sequence
MSTESMIRDVELAEEALPKKTGGPQGSRRCLFLSLFSFLIVAGATTLFCLLHFGVIGPQREEFPRDLSLISPLAQAVRSSSRTPSDKPVAHVVANPQAEGQLQWLNRRANALLANGVELRDNQLVVPSEGLYLIYSQVLFKGQGCPSTHVLLTHTISRIAVSYQTKVNLLSAIKSPCQRETPEGAEAKPWYEPIYLGGVFQLEKGDRLSAEINRPDYLDFAESGQVYFGIIAL
Host
Mouse
Reactivity
Human, Rat
Interspecies Antigen Sequence
Mouse (79); Rat (78)
Isotype
IgG1 kappa
Quality Control Testing
Antibody Reactive Against Recombinant Protein.
Western Blot detection against Immunogen (51.37 KDa) .
Storage Buffer
In 1x PBS, pH 7.4
Storage Instruction
Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot (Tissue lysate)
TNF monoclonal antibody (M02), clone S9. Western Blot analysis of TNF expression in rat testis.Western Blot (Recombinant protein)
Sandwich ELISA (Recombinant protein)
Detection limit for recombinant GST tagged TNF is approximately 0.03ng/ml as a capture antibody.ELISA
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Gene Info — TNF
Entrez GeneID
7124GeneBank Accession#
BC028148Protein Accession#
AAH28148Gene Name
TNF
Gene Alias
DIF, TNF-alpha, TNFA, TNFSF2
Gene Description
tumor necrosis factor (TNF superfamily, member 2)
Gene Ontology
HyperlinkGene Summary
This gene encodes a multifunctional proinflammatory cytokine that belongs to the tumor necrosis factor (TNF) superfamily. This cytokine is mainly secreted by macrophages. It can bind to, and thus functions through its receptors TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. This cytokine is involved in the regulation of a wide spectrum of biological processes including cell proliferation, differentiation, apoptosis, lipid metabolism, and coagulation. This cytokine has been implicated in a variety of diseases, including autoimmune diseases, insulin resistance, and cancer. Knockout studies in mice also suggested the neuroprotective function of this cytokine. [provided by RefSeq
Other Designations
APC1 protein|OTTHUMP00000029281|OTTHUMP00000037669|TNF superfamily, member 2|TNF, macrophage-derived|TNF, monocyte-derived|cachectin|tumor necrosis factor alpha
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Interactome
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Pathway
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Disease
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