SNAP25 293T Cell Transient Overexpression Lysate(Denatured)
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More Files
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Specification
Transfected Cell Line
293T
Plasmid
pCMV-SNAP25 full-length
Host
Human
Theoretical MW (kDa)
22.77
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-SNAP25 antibody (H00006616-B01) by Western Blots.
SDS-PAGE Gel
SNAP25 transfected lysate.
Western Blot
Lane 1: SNAP25 transfected lysate ( 22.77 KDa)
Lane 2: Non-transfected lysate.Storage Buffer
1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
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Gene Info — SNAP25
Entrez GeneID
6616GeneBank Accession#
NM_003081.2Protein Accession#
-Gene Name
SNAP25
Gene Alias
FLJ23079, RIC-4, RIC4, SEC9, SNAP, SNAP-25, bA416N4.2, dJ1068F16.2
Gene Description
synaptosomal-associated protein, 25kDa
Omim ID
600322Gene Ontology
HyperlinkGene Summary
Synaptic vesicle membrane docking and fusion is mediated by SNAREs (soluble N-ethylmaleimide-sensitive factor attachment protein receptors) located on the vesicle membrane (v-SNAREs) and the target membrane (t-SNAREs). The assembled v-SNARE/t-SNARE complex consists of a bundle of four helices, one of which is supplied by v-SNARE and the other three by t-SNARE. For t-SNAREs on the plasma membrane, the protein syntaxin supplies one helix and the protein encoded by this gene contributes the other two. Therefore, this gene product is a presynaptic plasma membrane protein involved in the regulation of neurotransmitter release. Two alternative transcript variants encoding different protein isoforms have been described for this gene. [provided by RefSeq
Other Designations
OTTHUMP00000030267|OTTHUMP00000030268|resistance to inhibitors of cholinesterase 4 homolog|synaptosomal-associated protein 25
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Interactome
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Pathway
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Disease
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