PCTK1 monoclonal antibody (M02), clone 2E5
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More Files
- More Functions
Western Blot (Transfected lysate)
Western Blot analysis of PCTK1 expression in transfected 293T cell line by PCTK1 monoclonal antibody (M02), clone 2E5.
Lane 1: PCTK1 transfected lysate (Predicted MW: 55.7 KDa).
Lane 2: Non-transfected lysate.
Immunoprecipitation
Immunoprecipitation of PCTK1 transfected lysate using anti-PCTK1 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with PCTK1 MaxPab rabbit polyclonal antibody.
Immunofluorescence
Immunofluorescence of monoclonal antibody to PCTK1 on HeLa cell . [antibody concentration 10 ug/ml]
Western Blot detection against Immunogen (34.21 KDa) .
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Specification
Product Description
Mouse monoclonal antibody raised against a partial recombinant PCTK1.
Immunogen
PCTK1 (AAH01048, 1 a.a. ~ 80 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Sequence
MDRMKKIKRQLSMTLRGGRGIDKTNGAPEQIGLDESGGGGGSDPGEAPTRAAPGELRSARGPLSSAPEIVHEDLKMGSDG
Host
Mouse
Reactivity
Human
Isotype
IgG2a Kappa
Quality Control Testing
Antibody Reactive Against Recombinant Protein.
Western Blot detection against Immunogen (34.21 KDa) .
Storage Buffer
In 1x PBS, pH 7.4
Storage Instruction
Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot (Transfected lysate)
Western Blot analysis of PCTK1 expression in transfected 293T cell line by PCTK1 monoclonal antibody (M02), clone 2E5.
Lane 1: PCTK1 transfected lysate (Predicted MW: 55.7 KDa).
Lane 2: Non-transfected lysate.
Western Blot (Recombinant protein)
Immunoprecipitation
Immunoprecipitation of PCTK1 transfected lysate using anti-PCTK1 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with PCTK1 MaxPab rabbit polyclonal antibody.ELISA
Immunofluorescence
Immunofluorescence of monoclonal antibody to PCTK1 on HeLa cell . [antibody concentration 10 ug/ml] -
Gene Info — PCTK1
Entrez GeneID
5127GeneBank Accession#
BC001048Protein Accession#
AAH01048Gene Name
PCTK1
Gene Alias
FLJ16665, PCTAIRE, PCTAIRE1, PCTGAIRE
Gene Description
PCTAIRE protein kinase 1
Omim ID
311550Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene belongs to the cdc2/cdkx subfamily of the ser/thr family of protein kinases. It may play a role in signal transduction cascades in terminally differentiated cells. This gene is thought to escape X inactivation. Alternative splicing results in two transcript variants encoding different isoforms. [provided by RefSeq
Other Designations
OTTHUMP00000023208|OTTHUMP00000023209|PCTAIRE-motif protein kinase 1|serine/threonine-protein kinase
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