MMP1 293T Cell Transient Overexpression Lysate(Denatured)
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Specification
Transfected Cell Line
293T
Plasmid
pCMV-MMP1 full-length
Host
Human
Theoretical MW (kDa)
54
Interspecies Antigen Sequence
Mouse (59); Rat (58)
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-MMP1 antibody (H00004312-B01) by Western Blots.
SDS-PAGE Gel
MMP1 transfected lysate
Western Blot
Lane 1: MMP1 transfected lysate ( 54 KDa).
Lane 2: Non-transfected lysate.Storage Buffer
1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
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Gene Info — MMP1
Entrez GeneID
4312GeneBank Accession#
NM_002421Protein Accession#
NP_002412Gene Name
MMP1
Gene Alias
CLG, CLGN
Gene Description
matrix metallopeptidase 1 (interstitial collagenase)
Gene Ontology
HyperlinkGene Summary
Proteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. Most MMP's are secreted as inactive proproteins which are activated when cleaved by extracellular proteinases. This gene encodes a secreted enzyme which breaks down the interstitial collagens, types I, II, and III. The gene is part of a cluster of MMP genes which localize to chromosome 11q22.3. Alternative splicing results in multiple transcript variants.[provided by RefSeq
Other Designations
fibroblast collagenase|interstitial collagenase|matrix metalloprotease 1|matrix metalloproteinase 1|matrix metalloproteinase 1 (interstitial collagenase)
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Interactome
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Pathway
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Disease
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