MET 293T Cell Transient Overexpression Lysate(Denatured)
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Specification
Transfected Cell Line
293T
Plasmid
pCMV-MET full-length
Host
Human
Theoretical MW (kDa)
155.5
Interspecies Antigen Sequence
Mouse (90); Rat (89)
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-MET antibody (H00004233-B01) by Western Blots.
SDS-PAGE Gel
MET transfected lysate.
Western Blot
Lane 1: MET transfected lysate ( 155.5 KDa)
Lane 2: Non-transfected lysate.Storage Buffer
1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
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Gene Info — MET
Entrez GeneID
4233GeneBank Accession#
NM_000245Protein Accession#
NP_000236Gene Name
MET
Gene Alias
AUTS9, HGFR, RCCP2, c-Met
Gene Description
met proto-oncogene (hepatocyte growth factor receptor)
Gene Ontology
HyperlinkGene Summary
The proto-oncogene MET product is the hepatocyte growth factor receptor and encodes tyrosine-kinase activity. The primary single chain precursor protein is post-translationally cleaved to produce the alpha and beta subunits, which are disulfide linked to form the mature receptor. Various mutations in the MET gene are associated with papillary renal carcinoma. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq
Other Designations
HGF receptor|OTTHUMP00000069168|SF receptor|met proto-oncogene|met proto-oncogene tyrosine kinase|oncogene MET|scatter factor receptor
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Interactome
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