IL4 293T Cell Transient Overexpression Lysate(Denatured)
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Specification
Transfected Cell Line
293T
Plasmid
pCMV-IL4 full-length
Host
Human
Theoretical MW (kDa)
16.94
Interspecies Antigen Sequence
Mouse (41); Rat (43)
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-IL4 antibody (H00003565-B01) by Western Blots.
Western Blot
Lane 1: IL4 transfected lysate ( 16.83 KDa)
Lane 2: Non-transfected lysate.SDS-PAGE Gel
IL4 transfected lysate.
Storage Buffer
1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
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Gene Info — IL4
Entrez GeneID
3565GeneBank Accession#
NM_000589Protein Accession#
NP_000580Gene Name
IL4
Gene Alias
BCGF-1, BCGF1, BSF1, IL-4, MGC79402
Gene Description
interleukin 4
Omim ID
147780Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is a pleiotropic cytokine produced by activated T cells. This cytokine is a ligand for interleukin 4 receptor. The interleukin 4 receptor also binds to IL13, which may contribute to many overlapping functions of this cytokine and IL13. STAT6, a signal transducer and activator of transcription, has been shown to play a central role in mediating the immune regulatory signal of this cytokine. This gene, IL3, IL5, IL13, and CSF2 form a cytokine gene cluster on chromosome 5q, with this gene particularly close to IL13. This gene, IL13 and IL5 are found to be regulated coordinately by several long-range regulatory elements in an over 120 kilobase range on the chromosome. Two alternatively spliced transcript variants of this gene encoding distinct isoforms have been reported. [provided by RefSeq
Other Designations
B cell growth factor 1|B_cell stimulatory factor 1|lymphocyte stimulatory factor 1
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