HSPD1 monoclonal antibody (M01), clone 2A7
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More Files
- More Functions
Western Blot (Transfected lysate)
Western Blot analysis of HSPD1 expression in transfected 293T cell line by HSPD1 monoclonal antibody (M01), clone 2A7.
Lane 1: HSPD1 transfected lysate (Predicted MW: 61.1 KDa).
Lane 2: Non-transfected lysate.
Sandwich ELISA (Recombinant protein)
Detection limit for recombinant GST tagged HSPD1 is 0.03 ng/ml as a capture antibody.
Western Blot detection against Immunogen (88.66 KDa) .
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Specification
Product Description
Mouse monoclonal antibody raised against a full-length recombinant HSPD1.
Immunogen
HSPD1 (AAH02676.1, 1 a.a. ~ 573 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Sequence
MLRLPTVFRQMRPVSRVLAPHLTRAYAKDVKFGADARALMLQGVDLLADAVAVTMGPKGRTVIIEQSWGSPKVTKDGVTVAKSIDLKDKYKNIGAKLVQDVANNTNEEAGDGTTTATVLARSIAKEGFEKISKGANPVEIRRGVMLAVDAVIAELKKQSKPVTTPEEIAQVATISANGDKEIGNIISDAMKKVGRKGVITVKDGKTLNDELEIIEGMKFDRGYISPYFINTSKGQKCEFQDAYVLLSEKKISSIQSIVPALEIANAHRKPLVIIAEDVDGEALSTLVLNRLKVGLQVVAVKAPGFGDNRKNQLKDMAIATGGAVFGEEGLTLNLEDVQPHDLGKVGEVIVTKDDAMLLKGKGDKAQIEKRIQEIIEQLDVTTSEYEKEKLNERLAKLSDGVAVLKVGGTSDVEVNEKKDRVTDALNATRAAVEEGIVLGGGCALLRCIPALDSLTPANEDQKIGIEIIKRTLKIPAMTIAKNAGVEGSLIVEKIMQSSSEVGYDAMAGDFVNMVEKGIIDPTKVVRTALLDAAGVASLLTTAEVVVTEIPKEEKDPGMGAMGGMGGGMGGGMF
Host
Mouse
Reactivity
Human
Interspecies Antigen Sequence
Mouse (98); Rat (98)
Isotype
IgG2b Kappa
Quality Control Testing
Antibody Reactive Against Recombinant Protein.
Western Blot detection against Immunogen (88.66 KDa) .
Storage Buffer
In 1x PBS, pH 7.4
Storage Instruction
Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot (Transfected lysate)
Western Blot analysis of HSPD1 expression in transfected 293T cell line by HSPD1 monoclonal antibody (M01), clone 2A7.
Lane 1: HSPD1 transfected lysate (Predicted MW: 61.1 KDa).
Lane 2: Non-transfected lysate.
Western Blot (Recombinant protein)
Sandwich ELISA (Recombinant protein)
Detection limit for recombinant GST tagged HSPD1 is 0.03 ng/ml as a capture antibody.ELISA
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Gene Info — HSPD1
Entrez GeneID
3329GeneBank Accession#
BC002676.2Protein Accession#
AAH02676.1Gene Name
HSPD1
Gene Alias
CPN60, GROEL, HLD4, HSP60, HSP65, HuCHA60, SPG13
Gene Description
heat shock 60kDa protein 1 (chaperonin)
Gene Ontology
HyperlinkGene Summary
This gene encodes a member of the chaperonin family. The encoded mitochondrial protein may function as a signaling molecule in the innate immune system. This protein is essential for the folding and assembly of newly imported proteins in the mitochondria. This gene is adjacent to a related family member and the region between the 2 genes functions as a bidirectional promoter. Two pseudogenes, both located on chromosome 8, have been associated with this gene. Two transcript variants encoding the same protein have been identified for this gene. Mutations associated with this gene cause autosomal recessive spastic paraplegia 13. [provided by RefSeq
Other Designations
P60 lymphocyte protein|chaperonin|heat shock 60kD protein 1 (chaperonin)|heat shock protein 65|mitochondrial heat shock 60kD protein 1 variant 1|mitochondrial matrix protein P1|short heat shock protein 60 Hsp60s1|spastic paraplegia 13 (autosomal dominant)
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Interactome
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Pathway
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Disease
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