FEN1 293T Cell Transient Overexpression Lysate(Denatured)
* The price is valid only in USA. Please select country.
-
More Files
- More Functions
Western Blot
Lane 1: FEN1 transfected lysate ( 41.8 KDa)
Lane 2: Non-transfected lysate.
SDS-PAGE Gel
FEN1 transfected lysate.
-
Specification
Transfected Cell Line
293T
Plasmid
pCMV-FEN1 full-length
Host
Human
Theoretical MW (kDa)
41.91
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-FEN1 antibody (H00002237-B01) by Western Blots.
Western Blot
Lane 1: FEN1 transfected lysate ( 41.8 KDa)
Lane 2: Non-transfected lysate.SDS-PAGE Gel
FEN1 transfected lysate.
Storage Buffer
1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
-
Applications
Western Blot
-
Gene Info — FEN1
Entrez GeneID
2237GeneBank Accession#
BC000323Protein Accession#
AAH00323Gene Name
FEN1
Gene Alias
FEN-1, MF1, RAD2
Gene Description
flap structure-specific endonuclease 1
Omim ID
600393Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene removes 5' overhanging flaps in DNA repair and processes the 5' ends of Okazaki fragments in lagging strand DNA synthesis. Direct physical interaction between this protein and AP endonuclease 1 during long-patch base excision repair provides coordinated loading of the proteins onto the substrate, thus passing the substrate from one enzyme to another. The protein is a member of the XPG/RAD2 endonuclease family and is one of ten proteins essential for cell-free DNA replication. DNA secondary structure can inhibit flap processing at certain trinucleotide repeats in a length-dependent manner by concealing the 5' end of the flap that is necessary for both binding and cleavage by the protein encoded by this gene. Therefore, secondary structure can deter the protective function of this protein, leading to site-specific trinucleotide expansions. [provided by RefSeq
Other Designations
DNase IV|maturation factor-1
-
Interactome
-
Pathway
-
Disease
- +1-909-264-1399
+1-909-992-0619
Toll Free : +1-877-853-6098 - +1-909-992-3401
- sales@abnova.com