FASN 293T Cell Transient Overexpression Lysate(Denatured)
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More Files
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Specification
Transfected Cell Line
293T
Plasmid
pCMV-FASN full-length
Host
Human
Theoretical MW (kDa)
48.4
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-FASN antibody (H00002194-B01) by Western Blots.
SDS-PAGE Gel
FASN transfected lysate.
Western Blot
Lane 1: FASN transfected lysate ( 48.4 KDa)
Lane 2: Non-transfected lysate.Storage Buffer
1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
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Gene Info — FASN
Entrez GeneID
2194GeneBank Accession#
BC007909.1Protein Accession#
-Gene Name
FASN
Gene Alias
FAS, MGC14367, MGC15706, OA-519, SDR27X1
Gene Description
fatty acid synthase
Omim ID
600212Gene Ontology
HyperlinkGene Summary
The enzyme encoded by this gene is a multifunctional protein. Its main function is to catalyze the synthesis of palmitate from acetyl-CoA and malonyl-CoA, in the presence of NADPH, into long-chain saturated fatty acids. In some cancer cell lines, this protein has been found to be fused with estrogen receptor-alpha (ER-alpha), in which the N-terminus of FAS is fused in-frame with the C-terminus of ER-alpha. [provided by RefSeq
Other Designations
short chain dehydrogenase/reductase family 27X, member 1
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Interactome
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Pathway
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Disease
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