AKR1C1 293T Cell Transient Overexpression Lysate(Denatured)
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More Files
- More Functions
SDS-PAGE Gel
AKR1C1 transfected lysate.
Western Blot
Lane 1: AKR1C1 transfected lysate ( 35.64 KDa)
Lane 2: Non-transfected lysate.
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Specification
Transfected Cell Line
293T
Plasmid
pCMV-AKR1C1 full-length
Host
Human
Theoretical MW (kDa)
35.64
Interspecies Antigen Sequence
Mouse (73); Rat (70)
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-AKR1C1 antibody (H00001645-B01) by Western Blots.
SDS-PAGE Gel
AKR1C1 transfected lysate.
Western Blot
Lane 1: AKR1C1 transfected lysate ( 35.64 KDa)
Lane 2: Non-transfected lysate.Storage Buffer
1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
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Gene Info — AKR1C1
Entrez GeneID
1645GeneBank Accession#
NM_001353.5Protein Accession#
NP_001344.2Gene Name
AKR1C1
Gene Alias
2-ALPHA-HSD, 20-ALPHA-HSD, C9, DD1, DDH, DDH1, H-37, HAKRC, MBAB, MGC8954
Gene Description
aldo-keto reductase family 1, member C1 (dihydrodiol dehydrogenase 1; 20-alpha (3-alpha)-hydroxysteroid dehydrogenase)
Omim ID
600449Gene Ontology
HyperlinkGene Summary
This gene encodes a member of the aldo/keto reductase superfamily, which consists of more than 40 known enzymes and proteins. These enzymes catalyze the conversion of aldehydes and ketones to their corresponding alcohols by utilizing NADH and/or NADPH as cofactors. The enzymes display overlapping but distinct substrate specificity. This enzyme catalyzes the reaction of progesterone to the inactive form 20-alpha-hydroxy-progesterone. This gene shares high sequence identity with three other gene members and is clustered with those three genes at chromosome 10p15-p14. [provided by RefSeq
Other Designations
20 alpha-hydroxysteroid dehydrogenase|OTTHUMP00000018992|aldo-keto reductase C|aldo-keto reductase family 1, member C1|chlordecone reductase homolog|dihydrodiol dehydrogenase 1|dihydrodiol dehydrogenase isoform DD1|hepatic dihydrodiol dehydrogenase|trans-
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Interactome
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Pathway
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Disease
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