ARSA 293T Cell Transient Overexpression Lysate(Denatured)
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More Files
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SDS-PAGE Gel
ARSA transfected lysate.
Western Blot
Lane 1: ARSA transfected lysate ( 53.6 KDa)
Lane 2: Non-transfected lysate.
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Specification
Transfected Cell Line
293T
Plasmid
pCMV-ARSA full-length
Host
Human
Theoretical MW (kDa)
53.6
Interspecies Antigen Sequence
Mouse (87); Rat (86)
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-ARSA antibody (H00000410-B01) by Western Blots.
SDS-PAGE Gel
ARSA transfected lysate.
Western Blot
Lane 1: ARSA transfected lysate ( 53.6 KDa)
Lane 2: Non-transfected lysate.Storage Buffer
1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
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Gene Info — ARSA
Entrez GeneID
410GeneBank Accession#
BC014210.2Protein Accession#
-Gene Name
ARSA
Gene Alias
MLD
Gene Description
arylsulfatase A
Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene hydrolyzes cerebroside sulfate to cerebroside and sulfate. Defects in this gene lead to metachromatic leucodystrophy (MLD), a progressive demyelination disease which results in a variety of neurological symptoms and ultimately death. Multiple alternatively spliced transcript variants, one of which encodes a distinct protein, have been described for this gene. [provided by RefSeq
Other Designations
OTTHUMP00000196546|OTTHUMP00000196548
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Interactome
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Pathway
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Disease
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