RHOA monoclonal antibody (M05), clone 1C1
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More Files
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Specification
Product Description
Mouse monoclonal antibody raised against a full length recombinant RHOA.
Immunogen
RHOA (AAH01360, 1 a.a. ~ 193 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Sequence
MAAIRKKLVIVGDGACGKTCLLIVFSKDQFPEVYVPTVFENYVADIEVDGKQVELALWDTAGQEDYDRLRPLSYPDTDVILMCFSIDSPDSLENIPEKWTPEVKHFCPNVPIILVGNKKDLRNDEHTRRELAKMKQEPVKPEEGRDMANRIGAFGYMECSAKTKDGVREVFEMATRAALQARRGKKKSGCLVL
Host
Mouse
Reactivity
Human
Isotype
IgG1 lambda
Quality Control Testing
Antibody Reactive Against Recombinant Protein.
Western Blot detection against Immunogen (46.97 KDa) .
Storage Buffer
In 1x PBS, pH 7.4
Storage Instruction
Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot (Cell lysate)
RHOA monoclonal antibody (M05), clone 1C1 Western Blot analysis of RHOA expression in HeLa ( Cat # L013V1 ).Western Blot (Transfected lysate)
Western Blot analysis of RHOA expression in transfected 293T cell line by RHOA monoclonal antibody (M05), clone 1C1.
Lane 1: RHOA transfected lysate(21.8 KDa).
Lane 2: Non-transfected lysate.
Western Blot (Recombinant protein)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunoperoxidase of monoclonal antibody to RHOA on formalin-fixed paraffin-embedded human tonsil tissue.[antibody concentration 5 ug/ml]Sandwich ELISA (Recombinant protein)
Detection limit for recombinant GST tagged RHOA is 3 ng/ml as a capture antibody.ELISA
In situ Proximity Ligation Assay (Cell)
Proximity Ligation Analysis of protein-protein interactions between MAPK8 and RHOA. HeLa cells were stained with anti-MAPK8 rabbit purified polyclonal 1:1200 and anti-RHOA mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue). -
Gene Info — RHOA
Entrez GeneID
387GeneBank Accession#
BC001360Protein Accession#
AAH01360Gene Name
RHOA
Gene Alias
ARH12, ARHA, RHO12, RHOH12
Gene Description
ras homolog gene family, member A
Omim ID
165390Gene Ontology
HyperlinkGene Summary
O
Other Designations
Aplysia ras-related homolog 12|oncogene RHO H12|small GTP binding protein RhoA
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Interactome
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Pathway
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Disease
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