APAF1 293T Cell Transient Overexpression Lysate(Denatured)
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More Files
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Specification
Transfected Cell Line
293T
Plasmid
pCMV-APAF1 full-length
Host
Human
Theoretical MW (kDa)
137.1
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-APAF1 antibody (H00000317-B01) by Western Blots.
SDS-PAGE Gel
APAF1 transfected lysate.
Western Blot
Lane 1: APAF1 transfected lysate ( 137.1 KDa)
Lane 2: Non-transfected lysate.Storage Buffer
1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
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Gene Info — APAF1
Entrez GeneID
317GeneBank Accession#
NM_181868Protein Accession#
NP_863658Gene Name
APAF1
Gene Alias
CED4, DKFZp781B1145
Gene Description
apoptotic peptidase activating factor 1
Omim ID
602233Gene Ontology
HyperlinkGene Summary
This gene encodes a cytoplasmic protein that initiates apoptosis. This protein contains several copies of the WD-40 domain, a caspase recruitment domain (CARD), and an ATPase domain (NB-ARC). Upon binding cytochrome c and dATP, this protein forms an oligomeric apoptosome. The apoptosome binds and cleaves caspase 9 preproprotein, releasing its mature, activated form. Activated caspase 9 stimulates the subsequent caspase cascade that commits the cell to apoptosis. Alternative splicing results in several transcript variants encoding different isoforms. [provided by RefSeq
Other Designations
apoptotic protease activating factor 1
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Interactome
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Pathway
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Disease
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