ALPL monoclonal antibody (M01), clone 4H1
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More Files
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Western Blot (Transfected lysate)
Western Blot analysis of ALPL expression in transfected 293T cell line by ALPL monoclonal antibody (M01), clone 4H1.
Lane 1: ALPL transfected lysate(57.3 KDa).
Lane 2: Non-transfected lysate.
Sandwich ELISA (Recombinant protein)
Detection limit for recombinant GST tagged ALPL is approximately 0.1ng/ml as a capture antibody.
RNAi Knockdown (Antibody validated)
Western blot analysis of ALPL over-expressed 293 cell line, cotransfected with ALPL Validated Chimera RNAi ( Cat # H00000249-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with ALPL monoclonal antibody (M01), clone 4H1 (Cat # H00000249-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.
Western Blot detection against Immunogen (37.73 KDa) .
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Specification
Product Description
Mouse monoclonal antibody raised against a partial recombinant ALPL.
Immunogen
ALPL (AAH21289.3, 29 a.a. ~ 138 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Sequence
WRDQAQETLKYALELQKLNTNVAKNVIMFLGDGMGVSTVTAARILKGQLHHNPGEETRLEMDKFPFVALSKTYNTNAQVPDSAGTATAYLCGVKANEGTVGVSAATERSR
Host
Mouse
Reactivity
Human
Interspecies Antigen Sequence
Mouse (95)
Isotype
IgG2b Kappa
Quality Control Testing
Antibody Reactive Against Recombinant Protein.
Western Blot detection against Immunogen (37.73 KDa) .
Storage Buffer
In 1x PBS, pH 7.4
Storage Instruction
Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot (Transfected lysate)
Western Blot analysis of ALPL expression in transfected 293T cell line by ALPL monoclonal antibody (M01), clone 4H1.
Lane 1: ALPL transfected lysate(57.3 KDa).
Lane 2: Non-transfected lysate.
Western Blot (Recombinant protein)
Sandwich ELISA (Recombinant protein)
Detection limit for recombinant GST tagged ALPL is approximately 0.1ng/ml as a capture antibody.ELISA
RNAi Knockdown (Antibody validated)
Western blot analysis of ALPL over-expressed 293 cell line, cotransfected with ALPL Validated Chimera RNAi ( Cat # H00000249-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with ALPL monoclonal antibody (M01), clone 4H1 (Cat # H00000249-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control. -
Gene Info — ALPL
Entrez GeneID
249GeneBank Accession#
BC021289Protein Accession#
AAH21289.3Gene Name
ALPL
Gene Alias
AP-TNAP, FLJ40094, FLJ93059, HOPS, MGC161443, MGC167935, TNAP, TNSALP
Gene Description
alkaline phosphatase, liver/bone/kidney
Gene Ontology
HyperlinkGene Summary
There are at least four distinct but related alkaline phosphatases: intestinal, placental, placental-like, and liver/bone/kidney (tissue non-specific). The first three are located together on chromosome 2, while the tissue non-specific form is located on chromosome 1. The product of this gene is a membrane bound glycosylated enzyme that is not expressed in any particular tissue and is, therefore, referred to as the tissue-nonspecific form of the enzyme. The exact physiological function of the alkaline phosphatases is not known. A proposed function of this form of the enzyme is matrix mineralization; however, mice that lack a functional form of this enzyme show normal skeletal development. This enzyme has been linked directly to hypophosphatasia, a disorder that is characterized by hypercalcemia and includes skeletal defects. The character of this disorder can vary, however, depending on the specific mutation since this determines age of onset and severity of symptoms. Alternatively spliced transcript variants, which encode the same protein, have been identified for this gene. [provided by RefSeq
Other Designations
OTTHUMP00000002971|OTTHUMP00000002972|alkaline phosphatase, tissue-nonspecific isozyme|alkaline phosphomonoesterase|glycerophosphatase|liver/bone/kidney-type alkaline phosphatase|tissue non-specific alkaline phosphatase|tissue-nonspecific ALP
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Interactome
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Pathway
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Disease
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Publication Reference
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An extended core nanocoax pillar architecture for enhanced molecular detection.
D'Imperio LA, Valera AE, Naughton JR, Archibald MM, Merlo JM, Connolly TJ, Burns MJ, Chiles TC, Naughton MJ.
Biosensors & Bioelectronics 2019 Jun; 134:83.
Application：Added, Recombinant protein.
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An extended core nanocoax pillar architecture for enhanced molecular detection.
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