AHSG monoclonal antibody (M02), clone 1D8
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More Files
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Specification
Product Description
Mouse monoclonal antibody raised against a full-length recombinant AHSG.
Immunogen
AHSG (AAH48198.1, 19 a.a. ~ 367 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Sequence
APHGPGLIYRQPNCDDPETEEAALVAIDYINQNLPWGYKHTLNQIDEVKVWPQQPSGELFEIEIDTLETTCHVLDPTPVARCSVRQLKEHAVEGDCDFQLLKLDGKFSVVYAKCDSSPDSAEDVRKVCQDCPLLAPLNDTRVVHAAKAALAAFNAQNNGSNFQLEEISRAQLVPLPPSTYVEFTVSGTDCVAKEATEAAKCNLLAEKQYGFCKATLSEKLGGAEVAVTCTVFQTQPVTSQPQPEGANEAVPTPVVDPDAPPSPPLGAPGLPPAGSPPDSHVLLAAPPGHQLHRAHYDLRHTFMGVVSLGSPSGEVSHPRKTRTVVQPSVGAAAGPVVPPCPGRIRHFKV
Host
Mouse
Reactivity
Human
Interspecies Antigen Sequence
Mouse (57)
Isotype
IgG1 Kappa
Quality Control Testing
Antibody Reactive Against Recombinant Protein.
Western Blot detection against Immunogen (64.13 KDa) .
Storage Buffer
In 1x PBS, pH 7.4
Storage Instruction
Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot (Transfected lysate)
Western Blot analysis of AHSG expression in transfected 293T cell line by AHSG monoclonal antibody (M02), clone 1D8.
Lane 1: AHSG transfected lysate(39.3 KDa).
Lane 2: Non-transfected lysate.
Western Blot (Recombinant protein)
Immunoprecipitation
Immunoprecipitation of AHSG transfected lysate using anti-AHSG monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with AHSG MaxPab rabbit polyclonal antibody.Sandwich ELISA (Recombinant protein)
Detection limit for recombinant GST tagged AHSG is 0.1 ng/ml as a capture antibody.ELISA
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Gene Info — AHSG
Entrez GeneID
197GeneBank Accession#
BC048198Protein Accession#
AAH48198.1Gene Name
AHSG
Gene Alias
A2HS, AHS, FETUA, HSGA
Gene Description
alpha-2-HS-glycoprotein
Omim ID
138680Gene Ontology
HyperlinkGene Summary
Alpha2-HS glycoprotein (AHSG), a glycoprotein present in the serum, is synthesized by hepatocytes. The AHSG molecule consists of two polypeptide chains, which are both cleaved from a proprotein encoded from a single mRNA. It is involved in several functions, such as endocytosis, brain development and the formation of bone tissue. The protein is commonly present in the cortical plate of the immature cerebral cortex and bone marrow hemopoietic matrix, and it has therefore been postulated that it participates in the development of the tissues. However, its exact significance is still obscure. [provided by RefSeq
Other Designations
Alpha-2HS-glycoprotein|fetuin-A
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Interactome
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Disease
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