ADAR purified MaxPab mouse polyclonal antibody (B01P)
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More Files
- More Functions
Western Blot (Tissue lysate)
ADAR MaxPab polyclonal antibody. Western Blot analysis of ADAR expression in human liver.
Western Blot (Cell lysate)
ADAR MaxPab polyclonal antibody. Western Blot analysis of ADAR expression in HepG2.
Western Blot (Transfected lysate)
Western Blot analysis of ADAR expression in transfected 293T cell line (H00000103-T01) by ADAR MaxPab polyclonal antibody.
Lane 1: ADAR transfected lysate(134.86 KDa).
Lane 2: Non-transfected lysate.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunoperoxidase of purified MaxPab antibody to ADAR on formalin-fixed paraffin-embedded human lung. [antibody concentration 3 ug/ml]
Immunofluorescence
Immunofluorescence of purified MaxPab antibody to ADAR on HeLa cell. [antibody concentration 10 ug/ml]
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Specification
Product Description
Mouse polyclonal antibody raised against a full-length human ADAR protein.
Immunogen
ADAR (P55265, 1 a.a. ~ 1226 a.a) full-length human protein.
Sequence
MNPRQGYSLSGYYTHPFQGYEHRQLRYQQPGPGSSPSSFLLKQIEFLKGQLPEAPVIGKQTPSLPPSLPGLRPRFPVLLASSTRGRQVDIRGVPRGVHLGSQGLQRGFQHPSPRGRSLPQRGVDCLSSHFQELSIYQDQEQRILKFLEELGEGKATTAHDLSGKLGTPKKEINRVLYSLAKKGKLQKEAGTPPLWKIAVSTQAWNQHSGVVRPDGHSQGAPNSDPSLEPEDRNSTSVSEDLLEPFIAVSAQAWNQHSGVVRPDSHSQGSPNSDPGLEPEDSNSTSALEDPLEFLDMAEIKEKICDYLFNVSDSSALNLAKNIGLTKARDINAVLIDMERQGDVYRQGTTPPIWHLTDKKRERMQIKRNTNSVPETAPAAIPETRRNAEFLTCNIPTSNASNNMVTTEKVENGQEPVIKLENRQEARPEPARLKPPVHYNGPSKAGYVDFENGQWATDDIPDDLNSIRAAPGEFRAIMEMPSFYSHGLPRCSPYKKLTECQLKNPISGLLEYAQFASQTCEFNMIEQSGPPHEPRFKFQVVINGREFPPAEAGSKKVAKQDAAMKAMTILLEEAKAKDSGKSEESSHYSTEKESEKTAESQTPTPSATSFFSGKSPVTTLLECMHKLGNSCEFRLLSKEGPAHEPKFQYCVAVGAQTFPSVSAPSKKVAKQMAAEEAMKALHGEATNSMASDNQPEGMISESLDNLESMMPNKVRKIGELVRYLNTNPVGGLLEYARSHGFAAEFKLVDQSGPPHEPKFVYQAKVGGRWFPAVCAHSKKQGKQEAADAALRVLIGENEKAERMGFTEVTPVTGASLRRTMLLLSRSPEAQPKTLPLTGSTFHDQIAMLSHRCFNTLTNSFQPSLLGRKILAAIIMKKDSEDMGVVVSLGTGNRCVKGDSLSLKGETVNDCHAEIISRRGFIRFLYSELMKYNSQTAKDSIFEPAKGGEKLQIKKTVSFHLYISTAPCGDGALFDKSCSDRAMESTESRHYPVFENPKQGKLRTKVENGEGTIPVESSDIVPTWDGIRLGERLRTMSCSDKILRWNVLGLQGALLTHFLQPIYLKSVTLGYLFSQGHLTRAICCRVTRDGSAFEDGLRHPFIVNHPKVGRVSIYDSKRQSGKTKETSVNWCLADGYDLEILDGTRGTVDGPRNELSRVSKKNIFLLFKKLCSFRYRRDLLRLSYGEAKKAARDYETAKNYFKKGLKDMGYGNWISKPQEEKNFYLCPV
Host
Mouse
Reactivity
Human
Interspecies Antigen Sequence
Mouse (74); Rat (76)
Quality Control Testing
Antibody reactive against mammalian transfected lysate.
Storage Buffer
In 1x PBS, pH 7.4
Storage Instruction
Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot (Tissue lysate)
ADAR MaxPab polyclonal antibody. Western Blot analysis of ADAR expression in human liver.Western Blot (Cell lysate)
ADAR MaxPab polyclonal antibody. Western Blot analysis of ADAR expression in HepG2.Western Blot (Transfected lysate)
Western Blot analysis of ADAR expression in transfected 293T cell line (H00000103-T01) by ADAR MaxPab polyclonal antibody.
Lane 1: ADAR transfected lysate(134.86 KDa).
Lane 2: Non-transfected lysate.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunoperoxidase of purified MaxPab antibody to ADAR on formalin-fixed paraffin-embedded human lung. [antibody concentration 3 ug/ml]Immunofluorescence
Immunofluorescence of purified MaxPab antibody to ADAR on HeLa cell. [antibody concentration 10 ug/ml] -
Gene Info — ADAR
Entrez GeneID
103GeneBank Accession#
P55265Protein Accession#
P55265Gene Name
ADAR
Gene Alias
ADAR1, DRADA, DSH, DSRAD, G1P1, IFI-4, IFI4, K88dsRBP, p136
Gene Description
adenosine deaminase, RNA-specific
Gene Ontology
HyperlinkGene Summary
This gene encodes the enzyme responsible for RNA editing by site-specific deamination of adenosines. This enzyme destabilizes double stranded RNA through conversion of adenosine to inosine. Mutations in this gene have been associated with dyschromatosis symmetrica hereditaria. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. [provided by RefSeq
Other Designations
136 kDa double-stranded RNA binding protein|OTTHUMP00000035372|OTTHUMP00000035373|adenosine deaminase acting on RNA 1-A|double-stranded RNA-specific adenosine deaminase|interferon-induced protein 4|interferon-inducible protein 4
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Interactome
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Pathway
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Disease
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Publication Reference
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ADAR2-Mediated Editing of miR-214 and miR-122 Precursor and Antisense RNA Transcripts in Liver Cancers.
Liu WH, Chen CH, Yeh KH, Li CL, Wu YJ, Chen DS, Chen PJ, Yeh SH.
Plos One 2013 Dec; 8(12):e81922.
Application：WB, Human, HepG2, Huh7 cells.
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ADAR2-Mediated Editing of miR-214 and miR-122 Precursor and Antisense RNA Transcripts in Liver Cancers.
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