mutaFISH™ CK20wt RNA Probes
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Specification
Product Description
mutaFISH™ CK20wt RNA Probes is designed to detect human CK20 gene on single strand RNA in cells using padlock probe and in situ rolling-circle amplification technology.
Reactivity
Human
Supplied Product
Content:
1. RT CK20 Primer
2. mutaFISH™ CK19wt RNA Probe
3. Detection Probe-FITCComparison
Fluorophore
FITC (Excitation Peak (nm): 495; Emission Peak (nm): 519)
Probe Position
Regulatory Status
For research use only (RUO)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
We recommend mutaFISH™ RNA Accessory Kit (Catalog #: KA4915) which provides necessary reagents and enzymes for in situ reverse transcription, RNA digestion, mutaFISH™ hybridization, ligation and amplication prior to mutaFISH™.
Video
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Applications
mutation specific, Fluorescence In Situ Hybridization (Cells)
mutaFISH™ staining was performed in situ in CK20 transfected 293T cells. CK20 gene was detected via green signal (FITC). -
Gene Info — KRT20
Entrez GeneID
54474Gene Name
KRT20
Gene Alias
CD20, CK20, K20, KRT21, MGC35423
Gene Description
keratin 20
Omim ID
608218Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is a member of the keratin family. The keratins are intermediate filament proteins responsible for the structural integrity of epithelial cells and are subdivided into cytokeratins and hair keratins. The type I cytokeratins consist of acidic proteins which are arranged in pairs of heterotypic keratin chains. This cytokeratin is a major cellular protein of mature enterocytes and goblet cells and is specifically expressed in the gastric and intestinal mucosa. The type I cytokeratin genes are clustered in a region of chromosome 17q12-q21. [provided by RefSeq
Other Designations
cytokeratin 20|keratin, type I cytoskeletal 20
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Interactome
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Disease
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