mutaFISH™ PD-L1wt RNA Probes
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More Files
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mutation specific, Fluorescence In Situ Hybridization (Cells)
Fig.1 mutaFISH™ staining was performed in situ in human H1975 cells. PD-L1 wildtype was detected via red signal (Cy5).
Fig.2 mutaFISH™ staining was performed in situ in human A549 cells. PD-L1 wildtype was not detected via red signal (Cy5).
mutation specific, Fluorescence In Situ Hybridization (FFPE Tissue)
mutaFISH™ staining was performed in situ in mouse FFPE PD-L1 293T tissue. PD-L1 gene was detected via red signal (Texas Red X).
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Specification
Product Description
mutaFISH™ PD-L1wt RNA Probes is designed to detect human PD-L1 gene on single strand RNA in cells using padlock probe and in situ rolling-circle amplification technology.
Reactivity
Human
Supplied Product
Content:
1. RT PD-L1 Primer
2. mutaFISH™ PD-L1wt RNA Probe
3. Detection Probe-Texas Red XComparison
Fluorophore
Texas Red X (Excitation Peak (nm): 595; Emission Peak 613)
Probe Position
Regulatory Status
For research use only (RUO)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
We recommend mutaFISH™ RNA Accessory Kit (Catalog #: KA4915) which provides necessary reagents and enzymes for in situ reverse transcription, RNA digestion, mutaFISH™ hybridization, ligation and amplication prior to mutaFISH™.
Video
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Applications
mutation specific, Fluorescence In Situ Hybridization (Cells)
Fig.1 mutaFISH™ staining was performed in situ in human H1975 cells. PD-L1 wildtype was detected via red signal (Cy5).
Fig.2 mutaFISH™ staining was performed in situ in human A549 cells. PD-L1 wildtype was not detected via red signal (Cy5).mutation specific, Fluorescence In Situ Hybridization (FFPE Tissue)
mutaFISH™ staining was performed in situ in mouse FFPE PD-L1 293T tissue. PD-L1 gene was detected via red signal (Texas Red X). -
Gene Info — CD274
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Interactome
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Pathway
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Disease
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