mutaFISH™ CEP1wt CEP7wt CEP8wt CEP17wt DNA Probes
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More Files
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mutation specific, Fluorescence In Situ Hybridization (Cells)
mutaFISH™ staining was performed in situ in human PBMC cells. CEP7 was detected via purple signal (Texas Red, recolored), CEP8 was detected via red signal (Cy5) and CEP17 was detected via orange signal (Cy3).
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Specification
Product Description
mutaFISH™ CEP1wt CEP7wt CEP8wt CEP17wt DNA Probes is designed to identify human CEP1, CEP7, CEP8, CEP17 amplification on dsDNA in cells using padlock probe and in situ rolling-circle amplification technology.
Reactivity
Human
Supplied Product
Content:
1. mutaFISH™ CEP1wt DNA Probe
2. mutaFISH™ CEP7wt DNA Probe
3. mutaFISH™ CEP8wt DNA Probe
4. mutaFISH™ CEP17wt DNA Probe
5. Detection Probe-FITC
6. Detection Probe-6-HEX
7. Detection Probe-Texas Red X
8. Detection Probe-Aqua 431Comparison
Fluorophore
FITC (Excitation Peak (nm): 495; Emission Peak (nm): 519)
6-HEX (Excitation Peak (nm): 533; Emission Peak (nm): 559)
Texas Red X (Excitation Peak (nm): 595; Emission Peak 613)
Aqua 431 (Excitation Peak (nm): 431; Emission Peak (nm): 480)Probe Position
Regulatory Status
For research use only (RUO)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
We recommend mutaFISH™ DNA Accessory Kit 2 for Cells (Catalog #: KA4928) which provides necessary reagents and enzymes for in situ restriction digestion, exonucleolysis, mutaFISH™ hybridization, ligation and amplification prior to mutaFISH™.
Video
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Applications
mutation specific, Fluorescence In Situ Hybridization (Cells)
mutaFISH™ staining was performed in situ in human PBMC cells. CEP7 was detected via purple signal (Texas Red, recolored), CEP8 was detected via red signal (Cy5) and CEP17 was detected via orange signal (Cy3).
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