mutaFISH™ AR-V7 ARwt RNA Probes
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Specification
Product Description
mutaFISH™ AR-V7 ARwt RNA Probes is designed to detect AR wildtype and AR-V7 on single strand RNA in cells using padlock probe and in situ rolling-circle amplification technology.
Reactivity
Human
Supplied Product
Content:
1. RT AR-V7 Primer
2. RT ARwt Primer
3. mutaFISH™ AR-V7 RNA Probe
4. mutaFISH™ ARwt RNA Probe
5. Detection Probe-Aqua 431
6. Detection Probe-Texas Red XComparison
Fluorophore
Aqua 431 (Excitation Peak (nm): 431; Emission Peak (nm): 480)
Texas Red X (Excitation Peak (nm): 595; Emission Peak 613)Probe Position
Regulatory Status
For research use only (RUO)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
We recommend mutaFISH™ RNA Accessory Kit (Catalog #: KA4915) which provides necessary reagents and enzymes for in situ reverse transcription, RNA digestion, mutaFISH™ hybridization, ligation and amplication prior to mutaFISH™.
Video
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Applications
mutation specific, Fluorescence In Situ Hybridization (Cells)
Fig.1 mutaFISH™ staining was performed in situ in human CWR22Rv1 cells captured by CytoQuest™ CR. AR-V7 was detected via orange signal (Cy3), and ARwt was detected via red signal (Cy5).
Fig.2 mutaFISH™ staining was performed in situ in human PBMC cells. Both AR-V7 and ARwt were not detected.mutation specific, Fluorescence In Situ Hybridization (Frozen Tissue)
mutaFISH™ staining was performed in situ in human frozen tissue from breast cancer. ARwt was detected via red signal (Cy5), and AR-V7 was detected via orange signal (Cy3). -
Gene Info — AR
Entrez GeneID
367Gene Name
AR
Gene Alias
AIS, DHTR, HUMARA, KD, NR3C4, SBMA, SMAX1, TFM
Gene Description
androgen receptor
Gene Ontology
HyperlinkGene Summary
The androgen receptor gene is more than 90 kb long and codes for a protein that has 3 major functional domains: the N-terminal domain, DNA-binding domain, and androgen-binding domain. The protein functions as a steroid-hormone activated transcription factor. Upon binding the hormone ligand, the receptor dissociates from accessory proteins, translocates into the nucleus, dimerizes, and then stimulates transcription of androgen responsive genes. This gene contains 2 polymorphic trinucleotide repeat segments that encode polyglutamine and polyglycine tracts in the N-terminal transactivation domain of its protein. Expansion of the polyglutamine tract causes spinal bulbar muscular atrophy (Kennedy disease). Mutations in this gene are also associated with complete androgen insensitivity (CAIS). Two alternatively spliced variants encoding distinct isoforms have been described. [provided by RefSeq
Other Designations
OTTHUMP00000023450|OTTHUMP00000061928|dihydrotestosterone receptor
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