CD45 Depletion Kit PLUS CSV enrichment antibody
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More Files
- More Functions
Immunofluorescence
Immunofluorescence staining performed on CTCs from the patient recoverd by SuperSlide™. CSV was detected via green signal (anti-CSV (FITC) monoclonal antibody).
WBC Depletion
Representative images of white blood cells before and after WBC depletion using CD45 Depletion Kit.
Cell Depletion Assay (IF)
Up to 2.7 log depletion of Jurkat cells
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Specification
Product Description
CD45 Depletion Kit PLUS CSV enrichment antibody for white blood cell depletion and cell-surface vimentin (CSV) positive cell enrichment.
Amenable Platform
Supplied Product
Kit content:
1. CD45 Magnetic AbBeads
2. RBC Lysis Buffer
3. CTC Buffer 1
4. CTC Buffer 2
5. Mouse Anti-CSV monoclonal antibody
*Reagents are sufficient for 16 assays using recommended protocol.Suitable Sample
Post densiity gradient blood sample
CD45 Magnetic AbBeads Specification
Particle Core: Superparamagnetic iron oxide
Antibody Functionalization: CD45 mouse monoclonal antibody; isotype: IgG1
Particle Size: 1 um
Storge buffer: PBS, pH 7.4 (0.1% BSA, 0.1% Tween 20, and 0.09% sodium azide)Available Test
16 assays
Quality Control Testing
Antibody Conjugation Yield (O.D. 280): 37.02 ug/mg beads
Cell Depletion Assay (IF)
Up to 2.7 log depletion of Jurkat cells
Storage Instruction
Store CD45 Magnetic AbBeads, RBC Lysis Buffer and CTC Buffer 2 at 2-8°C.
Store CTC Buffer 1 and Mouse Anti-CSV monoclonal antibody at -20°C.
Aliquot to avoid repeated freezing and thawing. -
Applications
Immunofluorescence
Immunofluorescence staining performed on CTCs from the patient recoverd by SuperSlide™. CSV was detected via green signal (anti-CSV (FITC) monoclonal antibody).WBC Depletion
Representative images of white blood cells before and after WBC depletion using CD45 Depletion Kit.
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