CCNA2 polyclonal antibody
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More Files
- More Functions
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining with CCNA2 polyclonal antibody (Cat # PAB9996) was diluted 1 : 500 to detect CCNA2 in human testis tissue. Tissue was formalin fixed and paraffin embedded. No pre-treatment of sample was required. The image shows the localization of antibody as the precipitated red signal, with a hematoxylin purple nuclear counter stain.
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of CCNA2.
Immunogen
A synthetic peptide corresponding to human CCNA2.
Host
Rabbit
Reactivity
Human, Mouse, Rat
Form
Liquid
Quality Control Testing
Antibody Reactive Against Synthetic Peptide.
Recommend Usage
ELISA (1:500-1:2000)
Western Blot (1:250-1:500)
The optimal working dilution should be determined by the end user.Storage Buffer
In antiserum (0.01% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining with CCNA2 polyclonal antibody (Cat # PAB9996) was diluted 1 : 500 to detect CCNA2 in human testis tissue. Tissue was formalin fixed and paraffin embedded. No pre-treatment of sample was required. The image shows the localization of antibody as the precipitated red signal, with a hematoxylin purple nuclear counter stain.Immunoprecipitation
Enzyme-linked Immunoabsorbent Assay
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Gene Info — CCNA2
Entrez GeneID
890Gene Name
CCNA2
Gene Alias
CCN1, CCNA
Gene Description
cyclin A2
Omim ID
123835Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance through the cell cycle. Cyclins function as regulators of CDK kinases. Different cyclins exhibit distinct expression and degradation patterns which contribute to the temporal coordination of each mitotic event. In contrast to cyclin A1, which is present only in germ cells, this cyclin is expressed in all tissues tested. This cyclin binds and activates CDC2 or CDK2 kinases, and thus promotes both cell cycle G1/S and G2/M transitions. [provided by RefSeq
Other Designations
cyclin A
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Interactome
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Pathway
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Disease
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Publication Reference
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Human cyclin A is adenovirus E1A-associated protein p60 and behaves differently from cyclin B.
Pines J, Hunter T.
Nature 1990 Aug; 346(6286):760.
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Hepatitis B virus integration in a cyclin A gene in a hepatocellular carcinoma.
Wang J, Chenivesse X, Henglein B, Brechot C.
Nature 1990 Feb; 343(6258):555.
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A 60 kd cdc2-associated polypeptide complexes with the E1A proteins in adenovirus-infected cells.
Giordano A, Whyte P, Harlow E, Franza BR Jr, Beach D, Draetta G.
Cell 1989 Sep; 58(5):981.
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Human cyclin A is adenovirus E1A-associated protein p60 and behaves differently from cyclin B.
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