CREB1 (phospho S133) polyclonal antibody (Cat # PAB9993) was used to detect phosphorylated CREB1 by immunoblot. Recombinant His-tagged human CREB1 was produced in E. coli and purified by metal affinity chromatography. An aliquot of purified CREB1 was phosphorylated in-vitro using Protein Kinase A and ATP. Immunoblot of control (-) and in-vitro phosphorylated CREB1 (+) was used to show that the antibody reacts specifically with the phosphorylated form. CREB1 polyclonal antibody (Cat # PAB9994) reacts equally with both non-phosphorylated and phosphorylated CREB1 (not shown). Detection occurs using a 1 : 500 dilution of antibody followed by 1 : 5,000 dilution of HRP Goat-a-Rabbit IgG with visualization via ECL. Film exposure was approximately 1'. Personal Communication, Boss, J., Emory University School of Medicine, Atlanta, GA.
Immunohistochemical staining of CREB1 (phospho S133) polyclonal antibody (Cat # PAB9993) was used at 20 ug/mL to detect signal in a variety of tissues including multi-human, multi-brain and multi-cancer slides. This image shows moderate to strong nuclear staining of tonsillar lymphocytes. Tissue was formalin-fixed and paraffin embedded. The image shows localization of the antibody as the precipitated red signal, with a hematoxylin purple nuclear counterstain. Personal Communication, Tina Roush, Life Span Biosciences, Seattle, WA.
This gene encodes a transcription factor that is a member of the leucine zipper family of DNA binding proteins. This protein binds as a homodimer to the cAMP-responsive element, an octameric palindrome. The protein is phosphorylated by several protein kinases, and induces transcription of genes in response to hormonal stimulation of the cAMP pathway. Alternate splicing of this gene results in two transcript variants encoding different isoforms. [provided by RefSeq
active transcription factor CREB,cAMP-response element-binding protein-1,transactivator protein