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Last updated: 2015/8/30
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CREB1 (phospho S133) polyclonal antibody

  • Catalog # : PAB9993
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  • Specification
  • Product Description:
  • Rabbit polyclonal antibody raised against synthetic phosphopeptide of CREB1.
  • Immunogen:
  • Synthetic phosphopeptide (conjugated with KLH) corresponding to residues surrounding S133 of human CREB1.
  • Host:
  • Rabbit
  • Reactivity:
  • Human, Mouse, Rat
  • Specificity:
  • This phospho specific polyclonal antibody is specific to phosphorylated pS133 of human CREB. Reactivity with non-phosphorylated CREB is minimal.
  • Form:
  • Liquid
  • Quality Control Testing:
  • Antibody Reactive Against Synthetic Peptide.
  • Recommend Usage:
  • ELISA (1:1000-1:6000)
    Western Blot (1:500-1:2000)
    Immunohistochemistry (20 ug/mL)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In 20 mM KH2PO4, 150 mM NaCl, pH 7.2 (0.01% sodium azide)
  • Storage Instruction:
  • Store at 4°C. For long term storage store at -20°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Publication Reference
  • Applications
  • Western Blot (Recombinant protein)
  • Western Blot (Recombinant protein)
  • CREB1 (phospho S133) polyclonal antibody (Cat # PAB9993) was used to detect phosphorylated CREB1 by immunoblot.
    Recombinant His-tagged human CREB1 was produced in E. coli and purified by metal affinity chromatography.
    An aliquot of purified CREB1 was phosphorylated in-vitro using Protein Kinase A and ATP.
    Immunoblot of control (-) and in-vitro phosphorylated CREB1 (+) was used to show that the antibody reacts specifically with the phosphorylated form.
    CREB1 polyclonal antibody (Cat # PAB9994) reacts equally with both non-phosphorylated and phosphorylated CREB1 (not shown).
    Detection occurs using a 1 : 500 dilution of antibody followed by 1 : 5,000 dilution of HRP Goat-a-Rabbit IgG with visualization via ECL.
    Film exposure was approximately 1'.
    Personal Communication, Boss, J., Emory University School of Medicine, Atlanta, GA.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemical staining of CREB1 (phospho S133) polyclonal antibody (Cat # PAB9993) was used at 20 ug/mL to detect signal in a variety of tissues including multi-human, multi-brain and multi-cancer slides.
    This image shows moderate to strong nuclear staining of tonsillar lymphocytes.
    Tissue was formalin-fixed and paraffin embedded.
    The image shows localization of the antibody as the precipitated red signal, with a hematoxylin purple nuclear counterstain.
    Personal Communication, Tina Roush, Life Span Biosciences, Seattle, WA.
  • ELISA
  • Application Image
  • Western Blot (Recombinant protein)
  • Western Blot (Recombinant protein)
  • enlarge
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • enlarge
  • ELISA
  • Gene Information
  • Entrez GeneID:
  • 1385
  • Gene Name:
  • CREB1
  • Gene Alias:
  • CREB,MGC9284
  • Gene Description:
  • cAMP responsive element binding protein 1
  • Gene Summary:
  • This gene encodes a transcription factor that is a member of the leucine zipper family of DNA binding proteins. This protein binds as a homodimer to the cAMP-responsive element, an octameric palindrome. The protein is phosphorylated by several protein kinases, and induces transcription of genes in response to hormonal stimulation of the cAMP pathway. Alternate splicing of this gene results in two transcript variants encoding different isoforms. [provided by RefSeq
  • Other Designations:
  • active transcription factor CREB,cAMP-response element-binding protein-1,transactivator protein
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