CREB1 (phospho S133) polyclonal antibody
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More Files
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Western Blot (Recombinant protein)
CREB1 (phospho S133) polyclonal antibody (Cat # PAB9993) was used to detect phosphorylated CREB1 by immunoblot.
Recombinant His-tagged human CREB1 was produced in E. coli and purified by metal affinity chromatography.
An aliquot of purified CREB1 was phosphorylated in-vitro using Protein Kinase A and ATP.
Immunoblot of control (-) and in-vitro phosphorylated CREB1 (+) was used to show that the antibody reacts specifically with the phosphorylated form.
CREB1 polyclonal antibody (Cat # PAB9994) reacts equally with both non-phosphorylated and phosphorylated CREB1 (not shown).
Detection occurs using a 1 : 500 dilution of antibody followed by 1 : 5,000 dilution of HRP Goat-a-Rabbit IgG with visualization via ECL.
Film exposure was approximately 1'.
Personal Communication, Boss, J., Emory University School of Medicine, Atlanta, GA.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining of CREB1 (phospho S133) polyclonal antibody (Cat # PAB9993) was used at 20 ug/mL to detect signal in a variety of tissues including multi-human, multi-brain and multi-cancer slides.
This image shows moderate to strong nuclear staining of tonsillar lymphocytes.
Tissue was formalin-fixed and paraffin embedded.
The image shows localization of the antibody as the precipitated red signal, with a hematoxylin purple nuclear counterstain.
Personal Communication, Tina Roush, Life Span Biosciences, Seattle, WA.
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic phosphopeptide of CREB1.
Immunogen
Synthetic phosphopeptide (conjugated with KLH) corresponding to residues surrounding S133 of human CREB1.
Host
Rabbit
Reactivity
Human, Mouse, Rat
Specificity
This phospho specific polyclonal antibody is specific to phosphorylated pS133 of human CREB. Reactivity with non-phosphorylated CREB is minimal.
Form
Liquid
Quality Control Testing
Antibody Reactive Against Synthetic Peptide.
Recommend Usage
ELISA (1:1000-1:6000)
Western Blot (1:500-1:2000)
Immunohistochemistry (20 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In 20 mM KH2PO4, 150 mM NaCl, pH 7.2 (0.01% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Recombinant protein)
CREB1 (phospho S133) polyclonal antibody (Cat # PAB9993) was used to detect phosphorylated CREB1 by immunoblot.
Recombinant His-tagged human CREB1 was produced in E. coli and purified by metal affinity chromatography.
An aliquot of purified CREB1 was phosphorylated in-vitro using Protein Kinase A and ATP.
Immunoblot of control (-) and in-vitro phosphorylated CREB1 (+) was used to show that the antibody reacts specifically with the phosphorylated form.
CREB1 polyclonal antibody (Cat # PAB9994) reacts equally with both non-phosphorylated and phosphorylated CREB1 (not shown).
Detection occurs using a 1 : 500 dilution of antibody followed by 1 : 5,000 dilution of HRP Goat-a-Rabbit IgG with visualization via ECL.
Film exposure was approximately 1'.
Personal Communication, Boss, J., Emory University School of Medicine, Atlanta, GA.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining of CREB1 (phospho S133) polyclonal antibody (Cat # PAB9993) was used at 20 ug/mL to detect signal in a variety of tissues including multi-human, multi-brain and multi-cancer slides.
This image shows moderate to strong nuclear staining of tonsillar lymphocytes.
Tissue was formalin-fixed and paraffin embedded.
The image shows localization of the antibody as the precipitated red signal, with a hematoxylin purple nuclear counterstain.
Personal Communication, Tina Roush, Life Span Biosciences, Seattle, WA.Enzyme-linked Immunoabsorbent Assay
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Gene Info — CREB1
Entrez GeneID
1385Gene Name
CREB1
Gene Alias
CREB, MGC9284
Gene Description
cAMP responsive element binding protein 1
Omim ID
123810Gene Ontology
HyperlinkGene Summary
This gene encodes a transcription factor that is a member of the leucine zipper family of DNA binding proteins. This protein binds as a homodimer to the cAMP-responsive element, an octameric palindrome. The protein is phosphorylated by several protein kinases, and induces transcription of genes in response to hormonal stimulation of the cAMP pathway. Alternate splicing of this gene results in two transcript variants encoding different isoforms. [provided by RefSeq
Other Designations
active transcription factor CREB|cAMP-response element-binding protein-1|transactivator protein
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Interactome
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Pathway
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Disease
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Publication Reference
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Distinct cAMP response element-binding protein (CREB) domains stimulate different steps in a concerted mechanism of transcription activation.
Kim J, Lu J, Quinn PG.
PNAS 2000 Oct; 97(21):11292.
Application：WB, Recombinant protein.
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MSK1 is required for CREB phosphorylation in response to mitogens in mouse embryonic stem cells.
Arthur JS, Cohen P.
FEBS Letters 2000 Sep; 482(1-2):44.
Application：WB, Mouse, Mouse embryonic stem cells.
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CREB regulates MHC class II expression in a CIITA-dependent manner.
Moreno CS, Beresford GW, Louis-Plence P, Morris AC, Boss JM.
Immunity 1999 Feb; 10(2):143.
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Distinct cAMP response element-binding protein (CREB) domains stimulate different steps in a concerted mechanism of transcription activation.
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