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Western blot analysis using CCNB1 polyclonal antibody (Cat # PAB9943) shows detection of CCNB1 present in asynchronous HeLa cell lysates.
Comparison to a molecular weight marker indicates a band of ~55 KDa corresponding to human CCNB1 (arrowhead).
Approximately 50 ug of lysate was loaded on to a 7% SDS-PAGE gel for separation.
After transfer to nitrocellulose, the blot was incubated with a 1 : 500 dilution of the antibody for 1 h at room temperature.
Detection occurred using a 1 : 10,000 of HRP conjugated Goat-a-Rabbit IgG.
Personal communication, Luca D'Agostino, Temple University, Philadelphia, PA.

Western blot analysis using CCNB1 polyclonal antibody (Cat # PAB9943) antibody shows detection of human CCNB1 present in asynchronous HN30 cell lysates.
HN30 cells are from head and neck cancer tumors that over express CCNB1 and D1.
Comparison to a molecular weight marker indicates a band of ~62 KDa corresponding to the expected molecular weight for the protein (arrowhead).
The blot was incubated with a 1 : 500 dilution of the antibody for 1 h at room temperature.
Detection occurred using a 1 : 10,000 of HRP conjugated Goat-a-Rabbit IgG and chemiluminescence reagent with a 1-min exposure time.
Personal communication, Luca Cote, Temple University, Philadelphia, PA.

Immunohistochemical staining with CCNB1 polyclonal antibody (Cat # PAB9943) was diluted 1 : 500 to detect CCNB1 in human brain cerebellum tissue.
Tissue was formalin fixed and paraffin embedded.
No pre-treatment of sample was required.
The image shows the localization of antibody as the precipitated red signal, with a hematoxylin purple nuclear counter stain.