AKT1 polyclonal antibody
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More Files
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Western Blot
Western blot using AKT1 polyclonal antibody (Cat # PAB9932) shows detection of endogenous protein in whole cell extracts from H2O2 treated MCF-7 cells (Lane 2).
Recombinant AKT1 is detected in lane 3.
Lanes 1 and 4 contain wcl from HeLa and A-431 cells, respectively.
Specific band staining is completely blocked when antibody is pre-incubated with the immunizing peptide (data not shown).
The band at ~55-60 KDa, indicated by the arrowhead, corresponds to the expected molecular weight of AKT1.
Primary antibody was diluted 1 : 300 in 1% BLOTTO and reacted with the membrane overnight at 4°C.
IRDYE800™ conjugated Dnky-a-Sheep IgG was used at a 1 : 10,000 dilution for 45 min at room temperature.
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Specification
Product Description
Sheep polyclonal antibody raised against synthetic peptide of AKT1.
Immunogen
A synthetic peptide corresponding to internal region of human AKT1.
Host
Sheep
Reactivity
Chicken, Human, Mouse, Rat
Form
Liquid
Quality Control Testing
Antibody Reactive Against Synthetic Peptide.
Recommend Usage
ELISA (1:5000-1:20000)
Western Blot (1:500-1:2000)
The optimal working dilution should be determined by the end user.Storage Buffer
In 20 mM KH2PO4, 150 mM NaCl, pH 7.2 (0.1% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot
Western blot using AKT1 polyclonal antibody (Cat # PAB9932) shows detection of endogenous protein in whole cell extracts from H2O2 treated MCF-7 cells (Lane 2).
Recombinant AKT1 is detected in lane 3.
Lanes 1 and 4 contain wcl from HeLa and A-431 cells, respectively.
Specific band staining is completely blocked when antibody is pre-incubated with the immunizing peptide (data not shown).
The band at ~55-60 KDa, indicated by the arrowhead, corresponds to the expected molecular weight of AKT1.
Primary antibody was diluted 1 : 300 in 1% BLOTTO and reacted with the membrane overnight at 4°C.
IRDYE800™ conjugated Dnky-a-Sheep IgG was used at a 1 : 10,000 dilution for 45 min at room temperature.Enzyme-linked Immunoabsorbent Assay
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Gene Info — AKT1
Entrez GeneID
207Protein Accession#
NP_005154;P31749Gene Name
AKT1
Gene Alias
AKT, MGC99656, PKB, PKB-ALPHA, PRKBA, RAC, RAC-ALPHA
Gene Description
v-akt murine thymoma viral oncogene homolog 1
Gene Ontology
HyperlinkGene Summary
The serine-threonine protein kinase encoded by the AKT1 gene is catalytically inactive in serum-starved primary and immortalized fibroblasts. AKT1 and the related AKT2 are activated by platelet-derived growth factor. The activation is rapid and specific, and it is abrogated by mutations in the pleckstrin homology domain of AKT1. It was shown that the activation occurs through phosphatidylinositol 3-kinase. In the developing nervous system AKT is a critical mediator of growth factor-induced neuronal survival. Survival factors can suppress apoptosis in a transcription-independent manner by activating the serine/threonine kinase AKT1, which then phosphorylates and inactivates components of the apoptotic machinery. Multiple alternatively spliced transcript variants have been found for this gene. [provided by RefSeq
Other Designations
RAC-alpha serine/threonine-protein kinase|murine thymoma viral (v-akt) oncogene homolog-1|protein kinase B|rac protein kinase alpha
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Interactome
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Pathway
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Disease
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Publication Reference
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BCR-tyrosine 177 plays an essential role in Ras and Akt activation and in human hematopoietic progenitor transformation in chronic myelogenous leukemia.
Chu S, Li L, Singh H, Bhatia R.
Cancer Research 2007 Jul; 67(14):7045.
Application：WB-Ce, Human, Human CD34 cells.
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Regulation of mammalian target of rapamycin activity in PTEN-inactive prostate cancer cells by I kappa B kinase alpha.
Dan HC, Adli M, Baldwin AS.
Cancer Research 2007 Jul; 67(13):6263.
Application：WB-Tr, Human, DU145, HeLa cells.
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AKT1 amplification regulates cisplatin resistance in human lung cancer cells through the mammalian target of rapamycin/p70S6K1 pathway.
Liu LZ, Zhou XD, Qian G, Shi X, Fang J, Jiang BH.
Cancer Research 2007 Jul; 67(13):6325.
Application：WB-Ce, Human, A-549 cells.
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BCR-tyrosine 177 plays an essential role in Ras and Akt activation and in human hematopoietic progenitor transformation in chronic myelogenous leukemia.
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