ABCB5 polyclonal antibody
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More Files
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Western Blot (Transfected lysate)
Western blot using ABCB5 polyclonal antibody (Cat # PAB9925) shows detection of ABCB5 beta in ~12.5 ug of transfected-Hi5 whole cell lysate.
No reaction was seen when antibody was pre-incubated with the immunizing peptide (data not shown).
A 3-8% Tris-acetate gel was used for separation.
The arrowhead corresponds to 117 kDa ABCB5.
The membrane was probed with the primary antibody at a 1 : 10,000 dilution in 5% milk in TBST at 4°C, overnight.
Personal Communication, JP Gillet, CCR-NCI, Bethesda, MD.
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of ABCB5.
Immunogen
A synthetic peptide corresponding to internal region of human ABCB5.
Host
Rabbit
Reactivity
Human
Form
Liquid
Quality Control Testing
Antibody Reactive Against Synthetic Peptide.
Recommend Usage
ELISA (1:80000-1:85000)
Western Blot (1:10000)
The optimal working dilution should be determined by the end user.Storage Buffer
In 20 mM KH2PO4, 150 mM NaCl, pH 7.2 (0.01% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Transfected lysate)
Western blot using ABCB5 polyclonal antibody (Cat # PAB9925) shows detection of ABCB5 beta in ~12.5 ug of transfected-Hi5 whole cell lysate.
No reaction was seen when antibody was pre-incubated with the immunizing peptide (data not shown).
A 3-8% Tris-acetate gel was used for separation.
The arrowhead corresponds to 117 kDa ABCB5.
The membrane was probed with the primary antibody at a 1 : 10,000 dilution in 5% milk in TBST at 4°C, overnight.
Personal Communication, JP Gillet, CCR-NCI, Bethesda, MD.Enzyme-linked Immunoabsorbent Assay
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Gene Info — ABCB5
Entrez GeneID
340273Protein Accession#
AAW31630;Q515Q7Gene Name
ABCB5
Gene Alias
ABCB5alpha, ABCB5beta, EST422562
Gene Description
ATP-binding cassette, sub-family B (MDR/TAP), member 5
Gene Ontology
HyperlinkGene Summary
ABCB5 belongs to the ATP-binding cassette (ABC) transporter superfamily of integral membrane proteins. These proteins participate in ATP-dependent transmembrane transport of structurally diverse molecules ranging from small ions, sugars, and peptides to more complex organic molecules (Chen et al., 2005 [PubMed 15760339]).[supplied by OMIM
Other Designations
ATP-binding cassette protein|ATP-binding cassette, sub-family B, member 5|P-glycoprotein ABCB5
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Pathway
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Disease
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Publication Reference
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Association between Tumorigenic Potential and the Fate of Cancer Cells in a Syngeneic Melanoma Model.
Krelin Y, Berkovich L, Amit M, Gil Z.
PLoS One 2013 Apr; 8(4):e62124.
Application：WB, Mouse, Human, Melanoma cells.
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ABCB5-mediated doxorubicin transport and chemoresistance in human malignant melanoma.
Frank NY, Margaryan A, Huang Y, Schatton T, Waaga-Gasser AM, Gasser M, Sayegh MH, Sadee W, Frank MH.
Cancer Research 2005 May; 65(10):4320.
Application：Func, IF, IHC, Flow Cyt, Human, Human malignant melanoma sections, G3361 cells.
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Principal expression of two mRNA isoforms (ABCB 5alpha and ABCB 5beta ) of the ATP-binding cassette transporter gene ABCB 5 in melanoma cells and melanocytes.
Chen KG, Szakacs G, Annereau JP, Rouzaud F, Liang XJ, Valencia JC, Nagineni CN, Hooks JJ, Hearing VJ, Gottesman MM.
Pigment Cell Research 2005 Apr; 18(2):102.
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Useful tool to generate unidirectional deletion vectors by utilizing the star activity of BamHI in an NcoI-BamHI-XhoI cassette.
Chen KG, Gottesman MM.
BioTechniques 2005 Feb; 38(2):198.
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Association between Tumorigenic Potential and the Fate of Cancer Cells in a Syngeneic Melanoma Model.
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