EGFR (phospho Y1172) polyclonal antibody
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More Files
- More Functions
Western Blot (Cell lysate)
Western blot analysis using EGFR (pY1172) polyclonal antibody (Cat # PAB8085) to detect EGFR in A-431 cell lysate, and A-431 cell treated with EGF 25 ng/ml for 15 or 30 min. Loading 50 ug total protein each well, and EGFR (pY1172) polyclonal antibody were diluted 1:1000.
Dot Blot (Peptide)
Dot blot analysis of EGFR (phospho Y1172) polyclonal antibody (Cat # PAB8085) on nitrocellulose membrane. 50 ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.5 ug/mL.
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic phosphopeptide of EGFR.
Immunogen
Synthetic phosphopeptide (conjugated with KLH) corresponding to residues surrounding Y1172 of human EGFR.
Host
Rabbit
Reactivity
Human
Form
Liquid
Purification
Protein A purification
Recommend Usage
ELISA (1:1000)
Dot Blot (1:500)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.09% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis using EGFR (pY1172) polyclonal antibody (Cat # PAB8085) to detect EGFR in A-431 cell lysate, and A-431 cell treated with EGF 25 ng/ml for 15 or 30 min. Loading 50 ug total protein each well, and EGFR (pY1172) polyclonal antibody were diluted 1:1000.Enzyme-linked Immunoabsorbent Assay
Dot Blot (Peptide)
Dot blot analysis of EGFR (phospho Y1172) polyclonal antibody (Cat # PAB8085) on nitrocellulose membrane. 50 ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.5 ug/mL. -
Gene Info — EGFR
Entrez GeneID
1956Protein Accession#
NP_005219;P00533Gene Name
EGFR
Gene Alias
ERBB, ERBB1, HER1, PIG61, mENA
Gene Description
epidermal growth factor receptor (erythroblastic leukemia viral (v-erb-b) oncogene homolog, avian)
Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is a transmembrane glycoprotein that is a member of the protein kinase superfamily. This protein is a receptor for members of the epidermal growth factor family. EGFR is a cell surface protein that binds to epidermal growth factor. Binding of the protein to a ligand induces receptor dimerization and tyrosine autophosphorylation and leads to cell proliferation. Mutations in this gene are associated with lung cancer. [provided by RefSeq
Other Designations
avian erythroblastic leukemia viral (v-erb-b) oncogene homolog|cell growth inhibiting protein 40|cell proliferation-inducing protein 61|epidermal growth factor receptor
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Interactome
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Pathway
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Disease
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Publication Reference
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A basic peptide within the juxtamembrane region is required for EGF receptor dimerization.
Aifa S, Aydin J, Nordvall G, Lundstrom I, Svensson SP, Hermanson O.
Experimental Cell Research 2005 Jan; 302(1):108.
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EGF signalling amplification induced by dynamic clustering of EGFR.
Ichinose J, Murata M, Yanagida T, Sako Y.
Biochemical and Biophysical Research Communications 2004 Nov; 324(3):1143.
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Signalling by the type 1 insulin-like growth factor receptor: interplay with the epidermal growth factor receptor.
Timothy E Adams, Neil M McKern, Colin W Ward.
Growth Factors 2004 Jun; 22(2):89.
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A basic peptide within the juxtamembrane region is required for EGF receptor dimerization.
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