NANOG polyclonal antibody
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More Files
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Specification
Product Description
Goat polyclonal antibody raised against synthetic peptide of NANOG.
Immunogen
A synthetic peptide corresponding to C-terminus of human NANOG.
Sequence
C-DLFLNYSMNMQPED
Host
Goat
Theoretical MW (kDa)
34.6
Reactivity
Human
Form
Liquid
Purification
Antigen affinity purification
Concentration
0.5 mg/mL
Recommend Usage
ELISA (1:128000)
Sandwich ELISA (5-10 ug/mL)
Western blot (0.05-0.1 ug/mL)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (1-2 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In Tris saline, pH 7.3 (0.5% BSA, 0.02% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
NANOG polyclonal antibody (Cat # PAB7232) (0.05 ug/mL) staining of HeLa (nuclear) cell lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
NANOG polyclonal antibody (Cat # PAB7232) (2 ug/mL) staining of paraffin embedded human testis. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.Enzyme-linked Immunoabsorbent Assay
Sandwich ELISA
NANOG polyclonal antibody (Cat # PAB7232) (5 ug/mL) as the reporter with the capture rabbit antibody (5 ug/mL). -
Gene Info — NANOG
Entrez GeneID
79923Protein Accession#
NP_079141.1Gene Name
NANOG
Gene Alias
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Gene Description
Nanog homeobox
Omim ID
607937Gene Ontology
HyperlinkOther Designations
homeobox transcription factor Nanog|homeobox transcription factor Nanog-delta 48
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Interactome
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Publication Reference
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NANOG maintains self-renewal of primate ES cells in the absence of a feeder layer.
Yasuda SY, Tsuneyoshi N, Sumi T, Hasegawa K, Tada T, Nakatsuji N, Suemori H.
Genes to Cells 2006 Sep; 11(9):1115.
Application:IF, WB, Monkey, CMK6, CMK9 cells.
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NANOG maintains self-renewal of primate ES cells in the absence of a feeder layer.
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