AIRE polyclonal antibody
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More Files
- More Functions
Western Blot (Tissue lysate)
AIRE polyclonal antibody (Cat # PAB7187) (0.3 ug/mL) staining of human spleen lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Western Blot (Transfected lysate)
AIRE polyclonal antibody (Cat # PAB7187) staining (0.05 ug/mL) of HEK293 cell lysates. Transfected with human AIRE (lane 1) and untransfected (Lane2). Data kindly provided by Prof. Part Peterson, University of Tartu, Estonia.
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Specification
Product Description
Goat polyclonal antibody raised against synthetic peptide of AIRE.
Immunogen
A synthetic peptide corresponding to human AIRE.
Sequence
C-KAKPPKKPESSAEQ
Host
Goat
Theoretical MW (kDa)
57.7, 36.5
Reactivity
Human, Mouse
Form
Liquid
Purification
Antigen affinity purification
Concentration
0.5 mg/mL
Quality Control Testing
Antibody Reactive Against Synthetic Peptide.
Recommend Usage
ELISA (1:64000)
Western Blot (0.3-1 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In Tris saline, pH 7.3 (0.5% BSA, 0.02% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Tissue lysate)
AIRE polyclonal antibody (Cat # PAB7187) (0.3 ug/mL) staining of human spleen lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.Western Blot (Transfected lysate)
AIRE polyclonal antibody (Cat # PAB7187) staining (0.05 ug/mL) of HEK293 cell lysates. Transfected with human AIRE (lane 1) and untransfected (Lane2). Data kindly provided by Prof. Part Peterson, University of Tartu, Estonia.Enzyme-linked Immunoabsorbent Assay
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Gene Info — AIRE
Entrez GeneID
326Protein Accession#
NP_000374.1;NP_000649.1Gene Name
AIRE
Gene Alias
AIRE1, APECED, APS1, APSI, PGA1
Gene Description
autoimmune regulator
Gene Ontology
HyperlinkGene Summary
This gene encodes a transcriptional regulator that forms nuclear bodies and interacts with the transcriptional coactivator CBP. At least three splice variant mRNAs products have been described including one which results in a premature stop codon and a transcript predicted to be a candidate for nuclear-mediated decay (NMD). Defects in this gene cause the rare autosomal-recessive systemic autoimmune disease termed autoimmune polyendocrinopathy-candidiasis-ectodermal dystrophy (APECED). [provided by RefSeq
Other Designations
autoimmune polyendocrinopathy candidiasis ectodermal dystrophy
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Interactome
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Pathway
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Disease
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Publication Reference
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Alteration of intra-pancreatic target-organ specificity by abrogation of Aire in NOD mice.
Niki S, Oshikawa K, Mouri Y, Hirota F, Matsushima A, Yano M, Han H, Bando Y, Izumi K, Matsumoto M, Nakayama KI, Kuroda N, Matsumoto M.
The Journal of Clinical Investigation 2006 May; 116(5):1292.
Application：IF, IHC, Mouse, Mouse thymus.
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Alteration of intra-pancreatic target-organ specificity by abrogation of Aire in NOD mice.
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